Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor

Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor

Despite the unprecedented gene editing capability of CRISPR-Cas9-mediated targeted knock-in, the efficiency and precision of this technology still require further optimization, particularly for multicellular model organisms, such as the zebrafish (Danio rerio). Our study demonstrated that an ∼200 ba...

Full description

Bibliographic Details
Main Authors: Deshani C. Ranawakage, Keita Okada, Kota Sugio, Yuya Kawaguchi, Yuki Kuninobu-Bonkohara, Takuya Takada, Yusuke Kamachi
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-02-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
CRISPR-Cas9
long ssDNA donor
knock-in
endogenous tagging
SoxB1
composite tag
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2020.598634/full

Internet

https://www.frontiersin.org/articles/10.3389/fcell.2020.598634/full

Similar Items