Determination of the Absolute Molar Mass of [Fe-S]-Containing Proteins Using Size Exclusion Chromatography-Multi-Angle Light Scattering (SEC-MALS)

Determination of the Absolute Molar Mass of [Fe-S]-Containing Proteins Using Size Exclusion Chromatography-Multi-Angle Light Scattering (SEC-MALS)

Size Exclusion Chromatography coupled with Multi-Angle Light Scattering (SEC-MALS) is a technique that determines the absolute molar mass (molecular weight) of macromolecules in solution, such as proteins or polymers, by detecting their light scattering intensity. Because SEC-MALS does not rely on t...

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Bibliographic Details
Main Authors: Christophe Velours, Jingjing Zhou, Paolo Zecchin, Nisha He, Myriam Salameh, Marie-Pierre Golinelli-Cohen, Béatrice Golinelli-Pimpaneau
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Biomolecules
Subjects:
SEC-MALS
size exclusion chromatography
multi-angle light scattering
molar mass
molecular weight
Fe-S cluster
Online Access:https://www.mdpi.com/2218-273X/12/2/270

Internet

https://www.mdpi.com/2218-273X/12/2/270

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