Facile means for quantifying microRNA expression by real-time PCR
MicroRNAs (miRNAs) are 20–24 nucleotide RNAs that are predicted to play regulatory roles in animals and plants. Here we report a simple and sensitive real-time PCR method for quantifying the expression of plant miRNAs. Total RNA, including miRNAs, was polyadenylated and reverse-transcribed with a po...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2005-10-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112010 |