Host DNA depletion can increase the sensitivity of Mycobacterium spp. detection through shotgun metagenomics in sputum

Host DNA depletion can increase the sensitivity of Mycobacterium spp. detection through shotgun metagenomics in sputum

Identification and phenotypic drug-susceptibility testing for mycobacteria are time-consuming and challenging but essential for managing mycobacterial infections. Next-generation sequencing (NGS) technologies can increase diagnostic speed and quality, but standardization is still lacking for many as...

Full description

Bibliographic Details
Main Authors: Nienke A. Kok, Nilay Peker, Leonard Schuele, Jessica L. de Beer, John W. A. Rossen, Bhanu Sinha, Natacha Couto
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-10-01
Series:Frontiers in Microbiology
Subjects:
internal transcribed spacer
N-acetyl-L-cysteine
next-generation sequencing
shotgun metagenomics
saponin treatment
tuberculosis
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.949328/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2022.949328/full

Similar Items