Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disorders
This study explores the implications of a novel germline missense mutation (R38C) in the succinate dehydrogenase (SDH) subunit B, which has been linked to neurodegenerative diseases. The mutation was identified from the SDH mutation database and corresponds to the SDH2R32C allele, mirroring the huma...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Molecular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1246842/full |
| _version_ | 1797671508900839424 |
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| author | Jiatong Zheng Siru Liu Dongdong Wang Linlin Li Surendra Sarsaiya Hua Zhou Heng Cai |
| author_facet | Jiatong Zheng Siru Liu Dongdong Wang Linlin Li Surendra Sarsaiya Hua Zhou Heng Cai |
| author_sort | Jiatong Zheng |
| collection | DOAJ |
| description | This study explores the implications of a novel germline missense mutation (R38C) in the succinate dehydrogenase (SDH) subunit B, which has been linked to neurodegenerative diseases. The mutation was identified from the SDH mutation database and corresponds to the SDH2R32C allele, mirroring the human SDHBR38C mutation. By subjecting the mutant yeast model to hydrogen peroxide (H2O2) stress, simulating oxidative stress, we observed heightened sensitivity to oxidative conditions. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed significant regulation (p < 0.05) of genes associated with antioxidant systems and energy metabolism. Through gas chromatography-mass spectrometry (GC-MS) analysis, we examined yeast cell metabolites under oxidative stress, uncovering insights into the potential protective role of o-vanillin. This study elucidates the biological mechanisms underlying cellular oxidative stress responses, offering valuable insights into its repercussions. These findings shed light on innovative avenues for addressing neurodegenerative diseases, potentially revolutionizing therapeutic strategies. |
| first_indexed | 2024-03-11T21:16:34Z |
| format | Article |
| id | doaj.art-0d00faca8c9a4a95a72ce5e31fa3a814 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-03-11T21:16:34Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-0d00faca8c9a4a95a72ce5e31fa3a8142023-09-28T17:48:40ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992023-09-011610.3389/fnmol.2023.12468421246842Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disordersJiatong ZhengSiru LiuDongdong WangLinlin LiSurendra SarsaiyaHua ZhouHeng CaiThis study explores the implications of a novel germline missense mutation (R38C) in the succinate dehydrogenase (SDH) subunit B, which has been linked to neurodegenerative diseases. The mutation was identified from the SDH mutation database and corresponds to the SDH2R32C allele, mirroring the human SDHBR38C mutation. By subjecting the mutant yeast model to hydrogen peroxide (H2O2) stress, simulating oxidative stress, we observed heightened sensitivity to oxidative conditions. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed significant regulation (p < 0.05) of genes associated with antioxidant systems and energy metabolism. Through gas chromatography-mass spectrometry (GC-MS) analysis, we examined yeast cell metabolites under oxidative stress, uncovering insights into the potential protective role of o-vanillin. This study elucidates the biological mechanisms underlying cellular oxidative stress responses, offering valuable insights into its repercussions. These findings shed light on innovative avenues for addressing neurodegenerative diseases, potentially revolutionizing therapeutic strategies.https://www.frontiersin.org/articles/10.3389/fnmol.2023.1246842/fullSaccharomyces cerevisiaesuccinate dehydrogenaseR32CqRT-PCRGC-MShydrogen peroxide stress |
| spellingShingle | Jiatong Zheng Siru Liu Dongdong Wang Linlin Li Surendra Sarsaiya Hua Zhou Heng Cai Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disorders Frontiers in Molecular Neuroscience Saccharomyces cerevisiae succinate dehydrogenase R32C qRT-PCR GC-MS hydrogen peroxide stress |
| title | Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disorders |
| title_full | Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disorders |
| title_fullStr | Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disorders |
| title_full_unstemmed | Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disorders |
| title_short | Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disorders |
| title_sort | unraveling the functional consequences of a novel germline missense mutation r38c in the yeast model of succinate dehydrogenase subunit b insights into neurodegenerative disorders |
| topic | Saccharomyces cerevisiae succinate dehydrogenase R32C qRT-PCR GC-MS hydrogen peroxide stress |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1246842/full |
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