CloneSeq - Single-cell clonal 3D culture and analysis protocol

CloneSeq - Single-cell clonal 3D culture and analysis protocol

Summary: This CloneSeq protocol combines clonal expansion inside 3D hydrogel spheres and droplet-based RNA sequencing to resolve the limited sensitivity of single-cell approaches. CloneSeq can reveal rare subpopulations and support cellular stemness. CloneSeq can be adapted to different biological s...

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Bibliographic Details
Main Authors: Xue Sun, Danny Bavli, Chen Kozulin, Alex Motzik, Amnon Buxboim, Oren Ram
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Sequence analysis
Cell Biology
Cell culture
Single Cell
Sequencing
RNAseq
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005001

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http://www.sciencedirect.com/science/article/pii/S2666166721005001

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