Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli

Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli

Abstract Background Shiga toxin-producing Escherichia coli (STEC), including E. coli O157:H7, are responsible for numerous foodborne outbreaks annually worldwide. E. coli O157:H7, as well as pathogenic non-O157:H7 STECs, can cause life-threating complications, such as bloody diarrhea (hemolytic coli...

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Bibliographic Details
Main Authors: Baoguang Li, Huanli Liu, Weimin Wang
Format: Article
Language:English
Published: BMC 2017-11-01
Series:BMC Microbiology
Subjects:
Multiplex real-time PCR
Escherichia coli O157:H7
Shiga toxins (stx1, stx2)
Shiga toxin-producing E. col (STEC)
non-O157
Limit of detection (LOD)
Online Access:http://link.springer.com/article/10.1186/s12866-017-1123-2

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http://link.springer.com/article/10.1186/s12866-017-1123-2

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