Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays

Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays

Summary: Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to T cell proliferation and differentiation into effector and memory cells. Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse...

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Bibliographic Details
Main Authors: Clémence Grosjean, Julie Quessada, Mathis Nozais, Marie Loosveld, Dominique Payet-Bornet, Cyrille Mionnet
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Cell-based Assays
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721006675

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http://www.sciencedirect.com/science/article/pii/S2666166721006675

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