A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin

A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin

Summary: Transposon insertion sequencing (TIS) is a highly effective method used with bacteria to identify genes important for growth in any condition of interest. Previously, we adapted this method to identify essential genes of the yeast Schizosaccharomyces pombe. Here, we describe modifications u...

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Bibliographic Details
Main Authors: Feng Li, Stevephen Hung, Caroline Esnault, Henry L. Levin
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Genomics
Sequencing
High-throughput screening
Model organisms
Molecular biology
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100099X

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http://www.sciencedirect.com/science/article/pii/S266616672100099X

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