A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin
Summary: Transposon insertion sequencing (TIS) is a highly effective method used with bacteria to identify genes important for growth in any condition of interest. Previously, we adapted this method to identify essential genes of the yeast Schizosaccharomyces pombe. Here, we describe modifications u...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672100099X |
| _version_ | 1819069144407998464 |
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| author | Feng Li Stevephen Hung Caroline Esnault Henry L. Levin |
| author_facet | Feng Li Stevephen Hung Caroline Esnault Henry L. Levin |
| author_sort | Feng Li |
| collection | DOAJ |
| description | Summary: Transposon insertion sequencing (TIS) is a highly effective method used with bacteria to identify genes important for growth in any condition of interest. Previously, we adapted this method to identify essential genes of the yeast Schizosaccharomyces pombe. Here, we describe modifications used to identify genes necessary for the formation of centromeric heterochromatin.For complete details on the use and execution of this protocol, please refer to Lee et al. (2020). |
| first_indexed | 2024-12-21T16:45:22Z |
| format | Article |
| id | doaj.art-0d63be7170034430b05235c2f6308d68 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-21T16:45:22Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-0d63be7170034430b05235c2f6308d682022-12-21T18:57:00ZengElsevierSTAR Protocols2666-16672021-06-0122100392A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatinFeng Li0Stevephen Hung1Caroline Esnault2Henry L. Levin3Division of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USADivision of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USABioinformatics and Scientific Programming Core, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USADivision of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding authorSummary: Transposon insertion sequencing (TIS) is a highly effective method used with bacteria to identify genes important for growth in any condition of interest. Previously, we adapted this method to identify essential genes of the yeast Schizosaccharomyces pombe. Here, we describe modifications used to identify genes necessary for the formation of centromeric heterochromatin.For complete details on the use and execution of this protocol, please refer to Lee et al. (2020).http://www.sciencedirect.com/science/article/pii/S266616672100099XGenomicsSequencingHigh-throughput screeningModel organismsMolecular biology |
| spellingShingle | Feng Li Stevephen Hung Caroline Esnault Henry L. Levin A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin STAR Protocols Genomics Sequencing High-throughput screening Model organisms Molecular biology |
| title | A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin |
| title_full | A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin |
| title_fullStr | A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin |
| title_full_unstemmed | A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin |
| title_short | A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin |
| title_sort | protocol for transposon insertion sequencing in schizosaccharomyces pombe to identify factors that maintain heterochromatin |
| topic | Genomics Sequencing High-throughput screening Model organisms Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S266616672100099X |
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