Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentillifera
This study screened several common economic algae to optimize the extraction process of β-1,3-xylan and analyze its monosaccharide composition. The extraction process of crude xylan was optimized by alkaline extraction method, and the monosaccharide composition of crude xylan was identified by a spe...
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| Format: | Article |
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The editorial department of Science and Technology of Food Industry
2023-08-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100086 |
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| author | Qian LIU Wenhui JIN Haotian JIAO Quanling XIE Yiping ZHANG Zhuan HONG Yuanhui ZHAO |
| author_facet | Qian LIU Wenhui JIN Haotian JIAO Quanling XIE Yiping ZHANG Zhuan HONG Yuanhui ZHAO |
| author_sort | Qian LIU |
| collection | DOAJ |
| description | This study screened several common economic algae to optimize the extraction process of β-1,3-xylan and analyze its monosaccharide composition. The extraction process of crude xylan was optimized by alkaline extraction method, and the monosaccharide composition of crude xylan was identified by a specific enzymatic method using Caulerpa lentillifera as the main raw material. The effect of extraction time, solid-liquid ratio and NaOH concentration on the yield of crude xylan was optimized with a single factor tests followed by three-three level orthogonal experiment to study the optimal extraction conditions of crude xylan. The results showed that the extraction rate of crude xylan was influenced by the following factors: Extraction time>material-to-liquid ratio>NaOH concentration. The optimum extraction process was: Extraction time of 3 h, NaOH concentration of 2.5 mol·L−1, feed-to-liquid ratio of 1:100 g·mL−1, and the yield of crude xylan was 28.1%±0.6%. The enzymatic products were determined by thin-layer chromatography, which showed that the crude xylan extracted from Caulerpa lentillifera was β-1,3-xylan and the monosaccharide fraction was β-1,3-xylose. |
| first_indexed | 2024-03-12T13:35:03Z |
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| id | doaj.art-0d93305d53214b03b0080c7940d6e2ad |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:35:03Z |
| publishDate | 2023-08-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-0d93305d53214b03b0080c7940d6e2ad2023-08-24T06:00:01ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-08-01441621021710.13386/j.issn1002-0306.20221000862022100086-16Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentilliferaQian LIU0Wenhui JIN1Haotian JIAO2Quanling XIE3Yiping ZHANG4Zhuan HONG5Yuanhui ZHAO6Technical Innovation Center for Utilization of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaTechnical Innovation Center for Utilization of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaTechnical Innovation Center for Utilization of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaTechnical Innovation Center for Utilization of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaTechnical Innovation Center for Utilization of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaTechnical Innovation Center for Utilization of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaSanya Institute of Oceanography, Ocean University of China, Sanya 572024, ChinaThis study screened several common economic algae to optimize the extraction process of β-1,3-xylan and analyze its monosaccharide composition. The extraction process of crude xylan was optimized by alkaline extraction method, and the monosaccharide composition of crude xylan was identified by a specific enzymatic method using Caulerpa lentillifera as the main raw material. The effect of extraction time, solid-liquid ratio and NaOH concentration on the yield of crude xylan was optimized with a single factor tests followed by three-three level orthogonal experiment to study the optimal extraction conditions of crude xylan. The results showed that the extraction rate of crude xylan was influenced by the following factors: Extraction time>material-to-liquid ratio>NaOH concentration. The optimum extraction process was: Extraction time of 3 h, NaOH concentration of 2.5 mol·L−1, feed-to-liquid ratio of 1:100 g·mL−1, and the yield of crude xylan was 28.1%±0.6%. The enzymatic products were determined by thin-layer chromatography, which showed that the crude xylan extracted from Caulerpa lentillifera was β-1,3-xylan and the monosaccharide fraction was β-1,3-xylose.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100086algaelcaulerpa lentilliferaβ-1,3-xylanalkali extractionextraction processβ-1,3-xylanase (xyl3088) |
| spellingShingle | Qian LIU Wenhui JIN Haotian JIAO Quanling XIE Yiping ZHANG Zhuan HONG Yuanhui ZHAO Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentillifera Shipin gongye ke-ji algael caulerpa lentillifera β-1,3-xylan alkali extraction extraction process β-1,3-xylanase (xyl3088) |
| title | Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentillifera |
| title_full | Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentillifera |
| title_fullStr | Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentillifera |
| title_full_unstemmed | Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentillifera |
| title_short | Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentillifera |
| title_sort | optimization of extraction process and analysis of monosaccharide composition of β 1 3 xylan from caulerpa lentillifera |
| topic | algael caulerpa lentillifera β-1,3-xylan alkali extraction extraction process β-1,3-xylanase (xyl3088) |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100086 |
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