Enhancing the Ability of Escherichia coli to Synthesise L-Isoleucine Using λ-Red Recombinant Technology Combined with Complex Mutagenesis

Enhancing the Ability of Escherichia coli to Synthesise L-Isoleucine Using λ-Red Recombinant Technology Combined with Complex Mutagenesis

To improve the synthetic ability of E. coli L-isoleucine by combining λ-Red recombination with complex mutagenesis. Firstly, taking E. coli NXA as the original strain, λ-Red homologous recombination was used to knock out the coding gene brnQ of branched chain amino acid transport protein to obtain m...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser: Jipeng WANG, Tengteng ZHU, Lu LIU, Cheng MA, Xiaobo WEI, Huiyan LIU, Haitian FANG
Format: Artikel
Sprache:zho
Veröffentlicht: The editorial department of Science and Technology of Food Industry 2025-01-01
Schriftenreihe:Shipin gongye ke-ji
Schlagworte:
escherichia coli
λ-red recombinant technology
brnq gene
complex mutagenesis
fermentation
l-isoleucine
Online Zugang:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020234

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http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020234

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