Enhancing the Ability of Escherichia coli to Synthesise L-Isoleucine Using λ-Red Recombinant Technology Combined with Complex Mutagenesis

To improve the synthetic ability of E. coli L-isoleucine by combining λ-Red recombination with complex mutagenesis. Firstly, taking E. coli NXA as the original strain, λ-Red homologous recombination was used to knock out the coding gene brnQ of branched chain amino acid transport protein to obtain m...

Popoln opis

Bibliografske podrobnosti
Main Authors: Jipeng WANG, Tengteng ZHU, Lu LIU, Cheng MA, Xiaobo WEI, Huiyan LIU, Haitian FANG
Format: Article
Jezik:zho
Izdano: The editorial department of Science and Technology of Food Industry 2025-01-01
Serija:Shipin gongye ke-ji
Teme:
Online dostop:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020234