Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with american cutaneous leishmaniasis

INTRODUCTION: The study analyzed positivity of polymerase chain reaction (PCR) on detection of DNA from Leishmania in patients' samples. METHODS: Extracted DNA was submitted to L150/L152, 13Y/13Z, and seminested PCR (snPCR). RESULTS: Results were evidenced by bands of approximately 120, 720, an...

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Main Authors: João Guilherme Lino da Silva, Thiago Miranda da Silva, Eduardo de Figueiredo Peloso, George Luiz Lins Machado-Coelho, Wilson Mayrink, Marília Caixeta Franco Ariosa, Paulo Márcio de Faria e Silva, Marcos José Marques
Format: Article
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2012-04-01
Series:Revista da Sociedade Brasileira de Medicina Tropical
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000200023&lng=en&tlng=en