Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with american cutaneous leishmaniasis

Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with american cutaneous leishmaniasis

INTRODUCTION: The study analyzed positivity of polymerase chain reaction (PCR) on detection of DNA from Leishmania in patients' samples. METHODS: Extracted DNA was submitted to L150/L152, 13Y/13Z, and seminested PCR (snPCR). RESULTS: Results were evidenced by bands of approximately 120, 720, an...

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Main Authors: João Guilherme Lino da Silva, Thiago Miranda da Silva, Eduardo de Figueiredo Peloso, George Luiz Lins Machado-Coelho, Wilson Mayrink, Marília Caixeta Franco Ariosa, Paulo Márcio de Faria e Silva, Marcos José Marques
Format: Article
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2012-04-01
Series:Revista da Sociedade Brasileira de Medicina Tropical
Subjects:
Leishmania
PCR
Diagnóstico
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000200023&lng=en&tlng=en

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