Protocol for generating embedding-free brain organoids enriched with oligodendrocytes

Summary: In response to the scarcity of advanced in vitro models dedicated to human CNS white matter research, we present a protocol to generate neuroectoderm-derived embedding-free human brain organoids enriched with oligodendrocytes. We describe steps for neuroectoderm differentiation, development...

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Bibliographic Details
Main Authors: Bahaa Al-mhanawi, Marta Boira Marti, Sean D. Morrison, Pallavi Gupta, Maath Alani, Peter G. Noakes, Ernst J. Wolvetang, Mohammed R. Shaker
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723006925