Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina

Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the tric...

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Main Authors: Tomasz Kulik, Maciej Buśko, Katarzyna Bilska, Anna Ostrowska-Kołodziejczak, Anne D. van Diepeningen, Juliusz Perkowski, Sebastian Stenglein
Format: Article
Language:English
Published: MDPI AG 2016-11-01
Series:Toxins
Subjects:
Online Access:http://www.mdpi.com/2072-6651/8/11/330
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author Tomasz Kulik
Maciej Buśko
Katarzyna Bilska
Anna Ostrowska-Kołodziejczak
Anne D. van Diepeningen
Juliusz Perkowski
Sebastian Stenglein
author_facet Tomasz Kulik
Maciej Buśko
Katarzyna Bilska
Anna Ostrowska-Kołodziejczak
Anne D. van Diepeningen
Juliusz Perkowski
Sebastian Stenglein
author_sort Tomasz Kulik
collection DOAJ
description Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.
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spelling doaj.art-0de45e7e03a94d35814bbfea9001b4932022-12-22T04:00:32ZengMDPI AGToxins2072-66512016-11-0181133010.3390/toxins8110330toxins8110330Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from ArgentinaTomasz Kulik0Maciej Buśko1Katarzyna Bilska2Anna Ostrowska-Kołodziejczak3Anne D. van Diepeningen4Juliusz Perkowski5Sebastian Stenglein6Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, Olsztyn 10-727, PolandDepartment of Chemistry, Poznań University of Life Sciences, ul. Wojska Polskiego 75, Poznań 60-625, PolandDepartment of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, Olsztyn 10-727, PolandDepartment of Chemistry, Poznań University of Life Sciences, ul. Wojska Polskiego 75, Poznań 60-625, PolandCBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, Utrecht 3584 CT, The NetherlandsDepartment of Chemistry, Poznań University of Life Sciences, ul. Wojska Polskiego 75, Poznań 60-625, PolandLaboratorio de Biología Funcional y Biotecnología (BIOLAB)-Comisión de Investigaciones Científicas de la provincia de Buenos Aires-Instituto de Investigaciones en Biodiversidad y Biotecnología-Consejo Nacional de Investigaciones Científicas y Técnicas (CICBA-INBIOTEC, CONICET), Av. República de Italia 780, Azul 7300, Buenos Aires, ArgentinaRecent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.http://www.mdpi.com/2072-6651/8/11/330FusariumTri genotypeschemotypes
spellingShingle Tomasz Kulik
Maciej Buśko
Katarzyna Bilska
Anna Ostrowska-Kołodziejczak
Anne D. van Diepeningen
Juliusz Perkowski
Sebastian Stenglein
Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina
Toxins
Fusarium
Tri genotypes
chemotypes
title Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina
title_full Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina
title_fullStr Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina
title_full_unstemmed Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina
title_short Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina
title_sort depicting the discrepancy between tri genotype and chemotype on the basis of strain cbs 139514 from a field population of f graminearum sensu stricto from argentina
topic Fusarium
Tri genotypes
chemotypes
url http://www.mdpi.com/2072-6651/8/11/330
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