A GC-MS/Single-Cell Method to Evaluate Membrane Transporter Substrate Specificity and Signaling

A GC-MS/Single-Cell Method to Evaluate Membrane Transporter Substrate Specificity and Signaling

Amino acid transporters play a vital role in metabolism and nutrient signaling pathways. Typically, transport activity is investigated using single substrates and competing amounts of other amino acids. We used GC-MS and LC-MS for metabolic screening of Xenopus laevis oocytes expressing various huma...

Full description

Bibliographic Details
Main Authors: Stephen J. Fairweather, Shoko Okada, Gregory Gauthier-Coles, Kiran Javed, Angelika Bröer, Stefan Bröer
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Molecular Biosciences
Subjects:
mTORC1 signaling
SNAT2
slc38a2
GC-MS
metabolomics
Xenopus laevis oocytes
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2021.646574/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2021.646574/full

Similar Items