Photo-Induced Depletion of Binding Sites in DNA-PAINT Microscopy

Photo-Induced Depletion of Binding Sites in DNA-PAINT Microscopy

The limited photon budget of fluorescent dyes is the main limitation for localization precision in localization-based super-resolution microscopy. Points accumulation for imaging in nanoscale topography (PAINT)-based techniques use the reversible binding of fluorophores and can sample a single bindi...

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Bibliographic Details
Main Authors: Philipp Blumhardt, Johannes Stein, Jonas Mücksch, Florian Stehr, Julian Bauer, Ralf Jungmann, Petra Schwille
Format: Article
Language:English
Published: MDPI AG 2018-11-01
Series:Molecules
Subjects:
DNA-PAINT
surface-integrated fluorescence correlation spectroscopy (SI-FCS)
reactive oxygen species
photo-induced DNA damage
super-resolution microscopy
Online Access:https://www.mdpi.com/1420-3049/23/12/3165

Internet

https://www.mdpi.com/1420-3049/23/12/3165

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