MSC stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cells
Abstract The tumor microenvironment enables important cellular interactions between cancer cells and recruited adjacent populations including mesenchymal stroma/stem cells (MSC). In vivo cellular interactions of primary human MSC in co-culture with human SK-OV-3 ovarian cancer cells revealed an incr...
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | Cell Communication and Signaling |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12964-018-0279-1 |
| _version_ | 1811210486084009984 |
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| author | Catharina Melzer Juliane von der Ohe Ralf Hass |
| author_facet | Catharina Melzer Juliane von der Ohe Ralf Hass |
| author_sort | Catharina Melzer |
| collection | DOAJ |
| description | Abstract The tumor microenvironment enables important cellular interactions between cancer cells and recruited adjacent populations including mesenchymal stroma/stem cells (MSC). In vivo cellular interactions of primary human MSC in co-culture with human SK-OV-3 ovarian cancer cells revealed an increased tumor growth as compared to mono-cultures of the ovarian cancer cells. Moreover, the presence of MSC stimulated formation of liver metastases. Further interactions of MSC with the ovarian cancer cells resulted in the formation of hybrid cells by cell fusion. Isolation and single cell cloning of these hybrid cells revealed two differentially fused ovarian cancer cell populations termed SK-hyb1 and SK-hyb2. RNA microarray analysis demonstrated expression profiles from both parental partners whereby SK-hyb1 were attributed with more SK-OV-3 like properties and SK-hyb2 cells displayed more similarities to MSC. Both ovarian cancer hybrid populations exhibited reduced proliferative capacity compared to the parental SK-OV-3 cells. Moreover, the fused populations failed to develop tumors in NODscid mice. Together, these data suggested certain stimulatory effects on ovarian tumor growth in the presence of MSC. Conversely, fusion of MSC with SK-OV-3 cells contributed to the generation of new cancer hybrid populations displaying a significantly reduced tumorigenicity. |
| first_indexed | 2024-04-12T04:56:31Z |
| format | Article |
| id | doaj.art-0e2b539b5c24467293b528ce5dca27c0 |
| institution | Directory Open Access Journal |
| issn | 1478-811X |
| language | English |
| last_indexed | 2024-04-12T04:56:31Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Communication and Signaling |
| spelling | doaj.art-0e2b539b5c24467293b528ce5dca27c02022-12-22T03:47:06ZengBMCCell Communication and Signaling1478-811X2018-10-011611910.1186/s12964-018-0279-1MSC stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cellsCatharina Melzer0Juliane von der Ohe1Ralf Hass2Biochemistry and Tumor Biology Lab, Department of Obstetrics and Gynecology (OE 6410), Hannover Medical SchoolBiochemistry and Tumor Biology Lab, Department of Obstetrics and Gynecology (OE 6410), Hannover Medical SchoolBiochemistry and Tumor Biology Lab, Department of Obstetrics and Gynecology (OE 6410), Hannover Medical SchoolAbstract The tumor microenvironment enables important cellular interactions between cancer cells and recruited adjacent populations including mesenchymal stroma/stem cells (MSC). In vivo cellular interactions of primary human MSC in co-culture with human SK-OV-3 ovarian cancer cells revealed an increased tumor growth as compared to mono-cultures of the ovarian cancer cells. Moreover, the presence of MSC stimulated formation of liver metastases. Further interactions of MSC with the ovarian cancer cells resulted in the formation of hybrid cells by cell fusion. Isolation and single cell cloning of these hybrid cells revealed two differentially fused ovarian cancer cell populations termed SK-hyb1 and SK-hyb2. RNA microarray analysis demonstrated expression profiles from both parental partners whereby SK-hyb1 were attributed with more SK-OV-3 like properties and SK-hyb2 cells displayed more similarities to MSC. Both ovarian cancer hybrid populations exhibited reduced proliferative capacity compared to the parental SK-OV-3 cells. Moreover, the fused populations failed to develop tumors in NODscid mice. Together, these data suggested certain stimulatory effects on ovarian tumor growth in the presence of MSC. Conversely, fusion of MSC with SK-OV-3 cells contributed to the generation of new cancer hybrid populations displaying a significantly reduced tumorigenicity.http://link.springer.com/article/10.1186/s12964-018-0279-1Mesenchymal stem cellsBreast and ovarian cancerTumor microenvironment |
| spellingShingle | Catharina Melzer Juliane von der Ohe Ralf Hass MSC stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cells Cell Communication and Signaling Mesenchymal stem cells Breast and ovarian cancer Tumor microenvironment |
| title | MSC stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cells |
| title_full | MSC stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cells |
| title_fullStr | MSC stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cells |
| title_full_unstemmed | MSC stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cells |
| title_short | MSC stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cells |
| title_sort | msc stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cells |
| topic | Mesenchymal stem cells Breast and ovarian cancer Tumor microenvironment |
| url | http://link.springer.com/article/10.1186/s12964-018-0279-1 |
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