Investigating the Assembly Status of the Plastid Encoded Polymerase Using BN-PAGE and Sucrose Gradient Centrifugation

The plastid encoded polymerase (PEP) represents a major transcription machinery in mature chloroplasts (Liere et al., 2011; Zhelyazkova et al., 2012). The proper assembly of this multi-subunit complex is important for plant growth and development (Pfalz and Pfannschmidt, 2013). The PEP polymerase ca...

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Bibliographic Details
Main Author: Jeannette Pfalz
Format: Article
Language:English
Published: Bio-protocol LLC 2016-07-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=1873&type=0