Prominin‐1 deletion results in spermatogenic impairment, sperm morphological defects, and infertility in mice
Abstract Purpose Spermatogenesis is a complex process orchestrated by several essential genes. Prominin‐1 (Prom1/PROM1) is a gene that is expressed in the testis but with a poorly understood role in spermatogenesis. Methods We used Prom1 knockout (Prom1 KO) mice to assess the role of Prom1 in sperma...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Reproductive Medicine and Biology |
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| Online Access: | https://doi.org/10.1002/rmb2.12514 |
| _version_ | 1797376181547302912 |
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| author | Haruka Matsukuma Yuka Kobayashi Shintaro Oka Fumiaki Higashijima Kazuhiro Kimura Erika Yoshihara Noriaki Sasai Koji Shiraishi |
| author_facet | Haruka Matsukuma Yuka Kobayashi Shintaro Oka Fumiaki Higashijima Kazuhiro Kimura Erika Yoshihara Noriaki Sasai Koji Shiraishi |
| author_sort | Haruka Matsukuma |
| collection | DOAJ |
| description | Abstract Purpose Spermatogenesis is a complex process orchestrated by several essential genes. Prominin‐1 (Prom1/PROM1) is a gene that is expressed in the testis but with a poorly understood role in spermatogenesis. Methods We used Prom1 knockout (Prom1 KO) mice to assess the role of Prom1 in spermatogenesis. To this end, we performed immunohistochemistry, immunofluorescence, western blotting, β‐galactosidase staining, and apoptosis assay. Additionally, we analyzed the morphology of sperm and assessed litter sizes. Results We observed that PROM1 is localized to the dividing spermatocytes in seminiferous epithelial cells, sperm, and columnar epithelium in the epididymis. In the Prom1 KO testis, an aberrant increase in apoptotic cells and a decrease in proliferating seminiferous epithelial cells were observed. Cellular FLICE‐like inhibitory protein (c‐FLIP) and extracellular signal‐regulated kinase 1/2 (ERK1/2) expression were also significantly decreased in Prom1 KO testis. In addition, a significantly increased number of epididymal spermatozoa with abnormal morphology and less motility was found in Prom1 KO mice. Conclusions PROM1 maintains spermatogenic cell proliferation and survival via c‐FLIP expression in the testis. It is also involved in sperm motility and fertilization potential. The mechanism underlying the effect of Prom1 on sperm morphology and motility remains to be identified. |
| first_indexed | 2024-03-08T19:35:00Z |
| format | Article |
| id | doaj.art-0e5f7c4851b34c3cadf5afab9f745068 |
| institution | Directory Open Access Journal |
| issn | 1445-5781 1447-0578 |
| language | English |
| last_indexed | 2024-03-08T19:35:00Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Reproductive Medicine and Biology |
| spelling | doaj.art-0e5f7c4851b34c3cadf5afab9f7450682023-12-26T04:30:45ZengWileyReproductive Medicine and Biology1445-57811447-05782023-01-01221n/an/a10.1002/rmb2.12514Prominin‐1 deletion results in spermatogenic impairment, sperm morphological defects, and infertility in miceHaruka Matsukuma0Yuka Kobayashi1Shintaro Oka2Fumiaki Higashijima3Kazuhiro Kimura4Erika Yoshihara5Noriaki Sasai6Koji Shiraishi7Department of Urology, School of Medicine Yamaguchi University Ube JapanDepartment of Ophthalmology, School of Medicine Yamaguchi University Ube JapanDepartment of Urology, School of Medicine Yamaguchi University Ube JapanDepartment of Ophthalmology, School of Medicine Yamaguchi University Ube JapanDepartment of Ophthalmology, School of Medicine Yamaguchi University Ube JapanDevelopmental Biomedical Science, Division of Biological Sciences Nara Institute of Science and Technology Ikoma Nara JapanDevelopmental Biomedical Science, Division of Biological Sciences Nara Institute of Science and Technology Ikoma Nara JapanDepartment of Urology, School of Medicine Yamaguchi University Ube JapanAbstract Purpose Spermatogenesis is a complex process orchestrated by several essential genes. Prominin‐1 (Prom1/PROM1) is a gene that is expressed in the testis but with a poorly understood role in spermatogenesis. Methods We used Prom1 knockout (Prom1 KO) mice to assess the role of Prom1 in spermatogenesis. To this end, we performed immunohistochemistry, immunofluorescence, western blotting, β‐galactosidase staining, and apoptosis assay. Additionally, we analyzed the morphology of sperm and assessed litter sizes. Results We observed that PROM1 is localized to the dividing spermatocytes in seminiferous epithelial cells, sperm, and columnar epithelium in the epididymis. In the Prom1 KO testis, an aberrant increase in apoptotic cells and a decrease in proliferating seminiferous epithelial cells were observed. Cellular FLICE‐like inhibitory protein (c‐FLIP) and extracellular signal‐regulated kinase 1/2 (ERK1/2) expression were also significantly decreased in Prom1 KO testis. In addition, a significantly increased number of epididymal spermatozoa with abnormal morphology and less motility was found in Prom1 KO mice. Conclusions PROM1 maintains spermatogenic cell proliferation and survival via c‐FLIP expression in the testis. It is also involved in sperm motility and fertilization potential. The mechanism underlying the effect of Prom1 on sperm morphology and motility remains to be identified.https://doi.org/10.1002/rmb2.12514flagellum functionProm1 KO miceProminin‐1sperm morphologyspermatogenesis |
| spellingShingle | Haruka Matsukuma Yuka Kobayashi Shintaro Oka Fumiaki Higashijima Kazuhiro Kimura Erika Yoshihara Noriaki Sasai Koji Shiraishi Prominin‐1 deletion results in spermatogenic impairment, sperm morphological defects, and infertility in mice Reproductive Medicine and Biology flagellum function Prom1 KO mice Prominin‐1 sperm morphology spermatogenesis |
| title | Prominin‐1 deletion results in spermatogenic impairment, sperm morphological defects, and infertility in mice |
| title_full | Prominin‐1 deletion results in spermatogenic impairment, sperm morphological defects, and infertility in mice |
| title_fullStr | Prominin‐1 deletion results in spermatogenic impairment, sperm morphological defects, and infertility in mice |
| title_full_unstemmed | Prominin‐1 deletion results in spermatogenic impairment, sperm morphological defects, and infertility in mice |
| title_short | Prominin‐1 deletion results in spermatogenic impairment, sperm morphological defects, and infertility in mice |
| title_sort | prominin 1 deletion results in spermatogenic impairment sperm morphological defects and infertility in mice |
| topic | flagellum function Prom1 KO mice Prominin‐1 sperm morphology spermatogenesis |
| url | https://doi.org/10.1002/rmb2.12514 |
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