Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi
Abstract Background The zoonotic simian parasite Plasmodium cynomolgi develops into replicating schizonts and dormant hypnozoites during the infection of hepatocytes and is used as a model organism to study relapsing malaria. The transcriptional profiling of P. cynomolgi liver stages was previously...
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | Malaria Journal |
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| Online Access: | https://doi.org/10.1186/s12936-022-04411-3 |
| _version_ | 1797977582303444992 |
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| author | Gabriel Mitchell Guglielmo Roma Annemarie Voorberg-van der Wel Martin Beibel Anne-Marie Zeeman Sven Schuierer Laura Torres Erika L. Flannery Clemens H. M. Kocken Sebastian A. Mikolajczak Thierry T. Diagana |
| author_facet | Gabriel Mitchell Guglielmo Roma Annemarie Voorberg-van der Wel Martin Beibel Anne-Marie Zeeman Sven Schuierer Laura Torres Erika L. Flannery Clemens H. M. Kocken Sebastian A. Mikolajczak Thierry T. Diagana |
| author_sort | Gabriel Mitchell |
| collection | DOAJ |
| description | Abstract Background The zoonotic simian parasite Plasmodium cynomolgi develops into replicating schizonts and dormant hypnozoites during the infection of hepatocytes and is used as a model organism to study relapsing malaria. The transcriptional profiling of P. cynomolgi liver stages was previously reported and revealed many important biological features of the parasite but left out the host response to malaria infection. Methods Previously published RNA sequencing data were used to quantify the expression of host genes in rhesus macaque hepatocytes infected with P . cynomolgi in comparison to either cells from uninfected samples or uninfected bystander cells. Results Although the dataset could not be used to resolve the transcriptional profile of hypnozoite-infected hepatocytes, it provided a snapshot of the host response to liver stage schizonts at 9–10 day post-infection and identified specific host pathways that are modulated during the exo-erythrocytic stage of P. cynomolgi. Conclusions This study constitutes a valuable resource characterizing the hepatocyte response to P. cynomolgi infection and provides a framework to build on future research that aims at understanding hepatocyte-parasite interactions during relapsing malaria infection. |
| first_indexed | 2024-04-11T05:09:18Z |
| format | Article |
| id | doaj.art-0e680a8fe8ac407888ecdc3feea9dfd8 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-04-11T05:09:18Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-0e680a8fe8ac407888ecdc3feea9dfd82022-12-25T12:06:00ZengBMCMalaria Journal1475-28752022-12-0121111210.1186/s12936-022-04411-3Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgiGabriel Mitchell0Guglielmo Roma1Annemarie Voorberg-van der Wel2Martin Beibel3Anne-Marie Zeeman4Sven Schuierer5Laura Torres6Erika L. Flannery7Clemens H. M. Kocken8Sebastian A. Mikolajczak9Thierry T. Diagana10Open Innovation at Novartis Institute for Tropical Diseases, Novartis Institutes for BioMedical ResearchChemical Biology & Therapeutics, Novartis Institutes for BioMedical ResearchDepartment of Parasitology, Biomedical Primate Research CentreChemical Biology & Therapeutics, Novartis Institutes for BioMedical ResearchDepartment of Parasitology, Biomedical Primate Research CentreChemical Biology & Therapeutics, Novartis Institutes for BioMedical ResearchOpen Innovation at Novartis Institute for Tropical Diseases, Novartis Institutes for BioMedical ResearchNovartis Institute for Tropical Diseases, Novartis Institutes for BioMedical ResearchDepartment of Parasitology, Biomedical Primate Research CentreNovartis Institute for Tropical Diseases, Novartis Institutes for BioMedical ResearchNovartis Institute for Tropical Diseases, Novartis Institutes for BioMedical ResearchAbstract Background The zoonotic simian parasite Plasmodium cynomolgi develops into replicating schizonts and dormant hypnozoites during the infection of hepatocytes and is used as a model organism to study relapsing malaria. The transcriptional profiling of P. cynomolgi liver stages was previously reported and revealed many important biological features of the parasite but left out the host response to malaria infection. Methods Previously published RNA sequencing data were used to quantify the expression of host genes in rhesus macaque hepatocytes infected with P . cynomolgi in comparison to either cells from uninfected samples or uninfected bystander cells. Results Although the dataset could not be used to resolve the transcriptional profile of hypnozoite-infected hepatocytes, it provided a snapshot of the host response to liver stage schizonts at 9–10 day post-infection and identified specific host pathways that are modulated during the exo-erythrocytic stage of P. cynomolgi. Conclusions This study constitutes a valuable resource characterizing the hepatocyte response to P. cynomolgi infection and provides a framework to build on future research that aims at understanding hepatocyte-parasite interactions during relapsing malaria infection.https://doi.org/10.1186/s12936-022-04411-3Host responseRelapsing malariaSchizontsHypnozoitesRNA sequencingLiver cells |
| spellingShingle | Gabriel Mitchell Guglielmo Roma Annemarie Voorberg-van der Wel Martin Beibel Anne-Marie Zeeman Sven Schuierer Laura Torres Erika L. Flannery Clemens H. M. Kocken Sebastian A. Mikolajczak Thierry T. Diagana Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi Malaria Journal Host response Relapsing malaria Schizonts Hypnozoites RNA sequencing Liver cells |
| title | Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi |
| title_full | Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi |
| title_fullStr | Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi |
| title_full_unstemmed | Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi |
| title_short | Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi |
| title_sort | transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite plasmodium cynomolgi |
| topic | Host response Relapsing malaria Schizonts Hypnozoites RNA sequencing Liver cells |
| url | https://doi.org/10.1186/s12936-022-04411-3 |
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