Heterologous expression and purification of encapsulins in Streptomyces coelicolor

In recent years a large number of encapsulin nanocompartment-encoding operons have been identified in bacterial and archaeal genomes. Encapsulin-encoding genes and operons from GC-rich Gram-positive bacteria, particularly of the phylum Actinobacteria, are often difficult to overexpress and purify in...

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Bibliographic Details
Main Authors: Michael P. Andreas, Tobias W. Giessen
Format: Article
Language:English
Published: Elsevier 2022-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016122001674