Establishment and evaluation of a multiplex real-time RT-PCR for quantitative and differential detection of wild-type canine distemper virus from vaccine strains

Establishment and evaluation of a multiplex real-time RT-PCR for quantitative and differential detection of wild-type canine distemper virus from vaccine strains

This study sought to establish a real-time reverse transcription (RT)-PCR method to differentially detect canine distemper virus (CDV) wild-type and vaccine strains. To this end, a pair of CDV universal primers and two specific minor groove binder (MGB) probes, harboring a T/C substitution in the he...

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Bibliographic Details
Main Authors: Ping Sui, Yiyang Sun, Yijun Shi, Wei Ran, Ning Shi, Dongbo Sun, Jiasan Zheng, Jianjun Zhao
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:Heliyon
Subjects:
Canine distemper virus
Wild-type strains
Vaccine strains
H gene
Real-time RT-PCR
Differential diagnosis
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844023065520

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http://www.sciencedirect.com/science/article/pii/S2405844023065520

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