Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway
Context: Chronic myeloid leukemia (CML) is a malignant hematopoietic stem cell disease caused by excessive proliferation and abnormal differentiation of hematopoietic stem cells. Asperuloside (ASP) is considered to have good biological activity and may be a good anti-CML drug. Objective: This study...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023107882 |
| _version_ | 1797336941494009856 |
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| author | Bingjie Zhao Hong Che Linlin Li Lian Hu Wenjing Yi Li Xiao Songshan Liu Zhufa Hou |
| author_facet | Bingjie Zhao Hong Che Linlin Li Lian Hu Wenjing Yi Li Xiao Songshan Liu Zhufa Hou |
| author_sort | Bingjie Zhao |
| collection | DOAJ |
| description | Context: Chronic myeloid leukemia (CML) is a malignant hematopoietic stem cell disease caused by excessive proliferation and abnormal differentiation of hematopoietic stem cells. Asperuloside (ASP) is considered to have good biological activity and may be a good anti-CML drug. Objective: This study aimed to explore the effects and possible mechanisms of ASP on the biological behavior of K562 cells based on RNA-seq. Materials and methods: The IC50 of ASP in K562 cells was calculated by the concentration-effect curve. Cell viability, apoptosis, and differentiation were detected by CCK8, flow cytometry, benzidine staining, and WB analysis, respectively. Further, RNA-seq was used to analyze the possible mechanism of ASP regulating K562 cells. Results: ASP significantly inhibited the proliferation, and promoted apoptosis and differentiation of K562 cells. A total of 117 differentially expressed genes were screened by RNA-seq, mainly involved in the RAS/MEK/ERK pathway. PD98059 was used to inhibit the RAS/MEK/ERK pathway in K562 cells, and results confirmed that PD98059 could not only inhibit the RAS/MEK/ERK pathway, but also inhibit the regulation of ASP on the proliferation and differentiation of K562 cells. Conclusion: ASP inhibited the proliferation, promoted apoptosis and differentiation of K562 cells by regulating the RAS/MEK/ERK pathway, and played a good anti-CML role. |
| first_indexed | 2024-03-08T09:02:09Z |
| format | Article |
| id | doaj.art-0e7d0816106a45958f909977fb477e4a |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T09:02:09Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-0e7d0816106a45958f909977fb477e4a2024-02-01T06:32:31ZengElsevierHeliyon2405-84402024-01-01101e23580Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathwayBingjie Zhao0Hong Che1Linlin Li2Lian Hu3Wenjing Yi4Li Xiao5Songshan Liu6Zhufa Hou7Department of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, ChinaDepartment of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, ChinaDepartment of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, ChinaDepartment of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, ChinaDepartment of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, ChinaDepartment of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, ChinaCorresponding author.; Department of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, ChinaCorresponding author.; Department of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, ChinaContext: Chronic myeloid leukemia (CML) is a malignant hematopoietic stem cell disease caused by excessive proliferation and abnormal differentiation of hematopoietic stem cells. Asperuloside (ASP) is considered to have good biological activity and may be a good anti-CML drug. Objective: This study aimed to explore the effects and possible mechanisms of ASP on the biological behavior of K562 cells based on RNA-seq. Materials and methods: The IC50 of ASP in K562 cells was calculated by the concentration-effect curve. Cell viability, apoptosis, and differentiation were detected by CCK8, flow cytometry, benzidine staining, and WB analysis, respectively. Further, RNA-seq was used to analyze the possible mechanism of ASP regulating K562 cells. Results: ASP significantly inhibited the proliferation, and promoted apoptosis and differentiation of K562 cells. A total of 117 differentially expressed genes were screened by RNA-seq, mainly involved in the RAS/MEK/ERK pathway. PD98059 was used to inhibit the RAS/MEK/ERK pathway in K562 cells, and results confirmed that PD98059 could not only inhibit the RAS/MEK/ERK pathway, but also inhibit the regulation of ASP on the proliferation and differentiation of K562 cells. Conclusion: ASP inhibited the proliferation, promoted apoptosis and differentiation of K562 cells by regulating the RAS/MEK/ERK pathway, and played a good anti-CML role.http://www.sciencedirect.com/science/article/pii/S2405844023107882RNA-seqIridoidPharmacological researchAnti-cancer |
| spellingShingle | Bingjie Zhao Hong Che Linlin Li Lian Hu Wenjing Yi Li Xiao Songshan Liu Zhufa Hou Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway Heliyon RNA-seq Iridoid Pharmacological research Anti-cancer |
| title | Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway |
| title_full | Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway |
| title_fullStr | Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway |
| title_full_unstemmed | Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway |
| title_short | Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway |
| title_sort | asperuloside regulates the proliferation apoptosis and differentiation of chronic myeloid leukemia cell line k562 through the ras mek erk pathway |
| topic | RNA-seq Iridoid Pharmacological research Anti-cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023107882 |
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