Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-between
The present article recapitulates the development of the polyhistidine affinity tag purification principle. Emphasis is laid on events behind the scenes which were never published. The key concept of the method emerged in a team discussion and its further development was driven by the need of Roche...
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2020-05-01
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| Series: | CHIMIA |
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| Online Access: | https://www.ingentaconnect.com/contentone/scs/chimia/2020/00000074/00000005/art00008 |