Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-between
The present article recapitulates the development of the polyhistidine affinity tag purification principle. Emphasis is laid on events behind the scenes which were never published. The key concept of the method emerged in a team discussion and its further development was driven by the need of Roche...
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2020-05-01
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| Series: | CHIMIA |
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| Online Access: | https://www.ingentaconnect.com/contentone/scs/chimia/2020/00000074/00000005/art00008 |
| _version_ | 1818419496441872384 |
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| author | Heinz Döbeli |
| author_facet | Heinz Döbeli |
| author_sort | Heinz Döbeli |
| collection | DOAJ |
| description | The present article recapitulates the development of the polyhistidine affinity tag purification principle. Emphasis is laid on events behind the scenes which were never published. The key concept of the method emerged in a team discussion and its further development was driven by the need of Roche in-house projects. |
| first_indexed | 2024-12-14T12:39:30Z |
| format | Article |
| id | doaj.art-0ea8f5243eff46a4936b5cdd39315995 |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-12-14T12:39:30Z |
| publishDate | 2020-05-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-0ea8f5243eff46a4936b5cdd393159952022-12-21T23:00:57ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242020-05-0174536336710.2533/chimia.2020.363Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-betweenHeinz Döbeli0Lupsingerstrasse 22, CH-4417 Ziefen, SwitzerlandThe present article recapitulates the development of the polyhistidine affinity tag purification principle. Emphasis is laid on events behind the scenes which were never published. The key concept of the method emerged in a team discussion and its further development was driven by the need of Roche in-house projects.https://www.ingentaconnect.com/contentone/scs/chimia/2020/00000074/00000005/art00008affinity tag removalhistidine affinity tagnickel chelateni-ntaprotein foldingprotein purification |
| spellingShingle | Heinz Döbeli Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-between CHIMIA affinity tag removal histidine affinity tag nickel chelate ni-nta protein folding protein purification |
| title | Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-between |
| title_full | Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-between |
| title_fullStr | Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-between |
| title_full_unstemmed | Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-between |
| title_short | Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-between |
| title_sort | polyhistidine affinity chromatography from the idea to a method of broad applicability and the pitfalls in between |
| topic | affinity tag removal histidine affinity tag nickel chelate ni-nta protein folding protein purification |
| url | https://www.ingentaconnect.com/contentone/scs/chimia/2020/00000074/00000005/art00008 |
| work_keys_str_mv | AT heinzdobeli polyhistidineaffinitychromatographyfromtheideatoamethodofbroadapplicabilityandthepitfallsinbetween |