Rapid Quantification of Gene Expression by Competitive RT-PCR and Ion-Pair Reversed-Phase HPLC
Competitive reverse-transcription PCR (RT-PCR) techniques for quantification of gene expression employ titrations in which the products of multiple PCRs must be separated, analyzed and quantified to compute gene expression in a single sample. We have employed a novel, ion-pair reversed-phase HPLC (I...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-02-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96202rr02 |