Ultracentrifugal separation, characterization, and functional study of extracellular vesicles derived from serum-free cell culture

Summary: Extracellular vesicles (EVs) play important roles in extracellular trafficking and signaling. Here, we separate EVs by differential centrifugation. EVs separated by this approach are called large EVs (l-EVs) and small EVs (s-EVs), reflecting particle size, which sediment based on different...

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Bibliographic Details
Main Authors: Hooi Ting Hu, Tamako Nishimura, Shiro Suetsugu
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003324