Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity.
HIV-1 infection dysregulates the immune system and alters gene expression in circulating monocytes. Differential gene expression analysis of CD14(+) monocytes from subjects infected with HIV-1 revealed increased expression of sialoadhesin (Sn, CD169, Siglec 1), a cell adhesion molecule first describ...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-04-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2288672?pdf=render |
| _version_ | 1819168450859237376 |
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| author | Hans Rempel Cyrus Calosing Bing Sun Lynn Pulliam |
| author_facet | Hans Rempel Cyrus Calosing Bing Sun Lynn Pulliam |
| author_sort | Hans Rempel |
| collection | DOAJ |
| description | HIV-1 infection dysregulates the immune system and alters gene expression in circulating monocytes. Differential gene expression analysis of CD14(+) monocytes from subjects infected with HIV-1 revealed increased expression of sialoadhesin (Sn, CD169, Siglec 1), a cell adhesion molecule first described in a subset of macrophages activated in chronic inflammatory diseases.We analyzed sialoadhesin expression on CD14(+) monocytes by flow cytometry and found significantly higher expression in subjects with elevated viral loads compared to subjects with undetectable viral loads. In cultured CD14(+) monocytes isolated from healthy individuals, sialoadhesin expression was induced by interferon-alpha and interferon-gamma but not tumor necrosis factor-alpha. Using a stringent binding assay, sialoadhesin-expressing monocytes adsorbed HIV-1 through interaction with the sialic acid residues on the viral envelope glycoprotein gp120. Furthermore, monocytes expressing sialoadhesin facilitated HIV-1 trans infection of permissive cells, which occurred in the absence of monocyte self-infection.Increased sialoadhesin expression on CD14(+) monocytes occurred in response to HIV-1 infection with maximum expression associated with high viral load. We show that interferons induce sialoadhesin in primary CD14(+) monocytes, which is consistent with an antiviral response during viremia. Our findings suggest that circulating sialoadhesin-expressing monocytes are capable of binding HIV-1 and effectively delivering virus to target cells thereby enhancing the distribution of HIV-1. Sialoadhesin could disseminate HIV-1 to viral reservoirs during monocyte immunosurveillance or migration to sites of inflammation and then facilitate HIV-1 infection of permissive cells. |
| first_indexed | 2024-12-22T19:03:48Z |
| format | Article |
| id | doaj.art-0ec089ce06f74a6cbca4e274ad978242 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T19:03:48Z |
| publishDate | 2008-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0ec089ce06f74a6cbca4e274ad9782422022-12-21T18:15:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-04-0134e196710.1371/journal.pone.0001967Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity.Hans RempelCyrus CalosingBing SunLynn PulliamHIV-1 infection dysregulates the immune system and alters gene expression in circulating monocytes. Differential gene expression analysis of CD14(+) monocytes from subjects infected with HIV-1 revealed increased expression of sialoadhesin (Sn, CD169, Siglec 1), a cell adhesion molecule first described in a subset of macrophages activated in chronic inflammatory diseases.We analyzed sialoadhesin expression on CD14(+) monocytes by flow cytometry and found significantly higher expression in subjects with elevated viral loads compared to subjects with undetectable viral loads. In cultured CD14(+) monocytes isolated from healthy individuals, sialoadhesin expression was induced by interferon-alpha and interferon-gamma but not tumor necrosis factor-alpha. Using a stringent binding assay, sialoadhesin-expressing monocytes adsorbed HIV-1 through interaction with the sialic acid residues on the viral envelope glycoprotein gp120. Furthermore, monocytes expressing sialoadhesin facilitated HIV-1 trans infection of permissive cells, which occurred in the absence of monocyte self-infection.Increased sialoadhesin expression on CD14(+) monocytes occurred in response to HIV-1 infection with maximum expression associated with high viral load. We show that interferons induce sialoadhesin in primary CD14(+) monocytes, which is consistent with an antiviral response during viremia. Our findings suggest that circulating sialoadhesin-expressing monocytes are capable of binding HIV-1 and effectively delivering virus to target cells thereby enhancing the distribution of HIV-1. Sialoadhesin could disseminate HIV-1 to viral reservoirs during monocyte immunosurveillance or migration to sites of inflammation and then facilitate HIV-1 infection of permissive cells.http://europepmc.org/articles/PMC2288672?pdf=render |
| spellingShingle | Hans Rempel Cyrus Calosing Bing Sun Lynn Pulliam Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity. PLoS ONE |
| title | Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity. |
| title_full | Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity. |
| title_fullStr | Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity. |
| title_full_unstemmed | Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity. |
| title_short | Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity. |
| title_sort | sialoadhesin expressed on ifn induced monocytes binds hiv 1 and enhances infectivity |
| url | http://europepmc.org/articles/PMC2288672?pdf=render |
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