Dexmedetomidine ameliorates hyperalgesia of rat models with neuropathic pain via upregulation of PPAR-γ
Objective To explore the analgesic effect and underlying mechanisms of dexmedetomidine(DEX) with spared nerve injury(SNI) rat model. Methods Forty-eight SD rats were randomly divided into control group, SNI group, DEX group and DEX+GW9662 group, with 12 rats in each. From first day to 14th days afte...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2023-09-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-9-1369.pdf |
| _version_ | 1797366245576671232 |
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| author | ZHU Feng, ZHONG Yue, DENG Yijiang, LIU Wenzhi, BIAN Jiang |
| author_facet | ZHU Feng, ZHONG Yue, DENG Yijiang, LIU Wenzhi, BIAN Jiang |
| author_sort | ZHU Feng, ZHONG Yue, DENG Yijiang, LIU Wenzhi, BIAN Jiang |
| collection | DOAJ |
| description | Objective To explore the analgesic effect and underlying mechanisms of dexmedetomidine(DEX) with spared nerve injury(SNI) rat model. Methods Forty-eight SD rats were randomly divided into control group, SNI group, DEX group and DEX+GW9662 group, with 12 rats in each. From first day to 14th days after SNI surgery, rats in DEX group were intra-thecally injected with 2 μg/kg (10 μL) of dexmedetomidine,rats in DEX group were intrathecally injected with 2 μg/kg of dexmedetomidine+300 μg of PPAR-γ inhibitor (GW9662), while rats in control and SNI groups were intrathecally injected with equal volume of solvent. At day 1 before operation, day 3, 7, 14 after operation, paw withdrawal threshold and thermal withdrawal latency were detected to evaluate the painintensity. At post-operative day 14,immunofluorescence staining was used to detect the expression of Iba-1 in spinal dorsal horn, Western blot was used to detect the expression of Iba-1 and PPAR-γ in ipsilateral spinal dorsal horn, ELISA was used to detect the levels of IFN-γ and IL-6 in ipsilateral spinal dorsal horn. Results On postoperative day 7 and 14, compared with control group, the pain intensity of ipsilateral hind paw and the expression of Iba-1, PPAR-γ, IL-6 and IFN-γ in ipsilateral spinal dorsal horn were enhanced in SNI group(P＜0.05). Compared with SNI group, the pain intensity of ipsilateral hind paw and the expression of Iba-1, PPAR-γ, IL-6 and IFN-γ in ipsilateral spinal dorsal horn decreased in EDX group(P＜0.05). Compared with DEX group, the pain intensity of ipsilateral hind paw and the expression of Iba-1, PPAR-γ, IL-6 and IFN-γ in ipsilateral spinal dorsal increased in DEX+GW9662 group(P＜0.05). Conclusions Dexmedetomidine obviously inhibits pain sensitization of SNI rats. The underlying analgesic mechanism may be related to the up-regulation of PPAR-γ in spinal dorsal horn. |
| first_indexed | 2024-03-08T17:02:37Z |
| format | Article |
| id | doaj.art-0ec12fcbe40c448e837536c24f77a26a |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T17:02:37Z |
| publishDate | 2023-09-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-0ec12fcbe40c448e837536c24f77a26a2024-01-04T07:33:35ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252023-09-014391369137410.16352/j.issn.1001-6325.2023.09.1369Dexmedetomidine ameliorates hyperalgesia of rat models with neuropathic pain via upregulation of PPAR-γZHU Feng, ZHONG Yue, DENG Yijiang, LIU Wenzhi, BIAN Jiang01. Department of Anesthesiology, Affiliated Hospital of Panzhihua University, Panzhihua, 617000; ;2. Department of Anesthesiology, Panzhihua Central Hospital, Panzhihua 617000, ChinaObjective To explore the analgesic effect and underlying mechanisms of dexmedetomidine(DEX) with spared nerve injury(SNI) rat model. Methods Forty-eight SD rats were randomly divided into control group, SNI group, DEX group and DEX+GW9662 group, with 12 rats in each. From first day to 14th days after SNI surgery, rats in DEX group were intra-thecally injected with 2 μg/kg (10 μL) of dexmedetomidine,rats in DEX group were intrathecally injected with 2 μg/kg of dexmedetomidine+300 μg of PPAR-γ inhibitor (GW9662), while rats in control and SNI groups were intrathecally injected with equal volume of solvent. At day 1 before operation, day 3, 7, 14 after operation, paw withdrawal threshold and thermal withdrawal latency were detected to evaluate the painintensity. At post-operative day 14,immunofluorescence staining was used to detect the expression of Iba-1 in spinal dorsal horn, Western blot was used to detect the expression of Iba-1 and PPAR-γ in ipsilateral spinal dorsal horn, ELISA was used to detect the levels of IFN-γ and IL-6 in ipsilateral spinal dorsal horn. Results On postoperative day 7 and 14, compared with control group, the pain intensity of ipsilateral hind paw and the expression of Iba-1, PPAR-γ, IL-6 and IFN-γ in ipsilateral spinal dorsal horn were enhanced in SNI group(P＜0.05). Compared with SNI group, the pain intensity of ipsilateral hind paw and the expression of Iba-1, PPAR-γ, IL-6 and IFN-γ in ipsilateral spinal dorsal horn decreased in EDX group(P＜0.05). Compared with DEX group, the pain intensity of ipsilateral hind paw and the expression of Iba-1, PPAR-γ, IL-6 and IFN-γ in ipsilateral spinal dorsal increased in DEX+GW9662 group(P＜0.05). Conclusions Dexmedetomidine obviously inhibits pain sensitization of SNI rats. The underlying analgesic mechanism may be related to the up-regulation of PPAR-γ in spinal dorsal horn.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-9-1369.pdfdexmedetomidine|neuropathic pain|microglia|peroxisome proliferator-activated receptor-γ(ppar-γ) |
| spellingShingle | ZHU Feng, ZHONG Yue, DENG Yijiang, LIU Wenzhi, BIAN Jiang Dexmedetomidine ameliorates hyperalgesia of rat models with neuropathic pain via upregulation of PPAR-γ Jichu yixue yu linchuang dexmedetomidine|neuropathic pain|microglia|peroxisome proliferator-activated receptor-γ(ppar-γ) |
| title | Dexmedetomidine ameliorates hyperalgesia of rat models with neuropathic pain via upregulation of PPAR-γ |
| title_full | Dexmedetomidine ameliorates hyperalgesia of rat models with neuropathic pain via upregulation of PPAR-γ |
| title_fullStr | Dexmedetomidine ameliorates hyperalgesia of rat models with neuropathic pain via upregulation of PPAR-γ |
| title_full_unstemmed | Dexmedetomidine ameliorates hyperalgesia of rat models with neuropathic pain via upregulation of PPAR-γ |
| title_short | Dexmedetomidine ameliorates hyperalgesia of rat models with neuropathic pain via upregulation of PPAR-γ |
| title_sort | dexmedetomidine ameliorates hyperalgesia of rat models with neuropathic pain via upregulation of ppar γ |
| topic | dexmedetomidine|neuropathic pain|microglia|peroxisome proliferator-activated receptor-γ(ppar-γ) |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-9-1369.pdf |
| work_keys_str_mv | AT zhufengzhongyuedengyijiangliuwenzhibianjiang dexmedetomidineameliorateshyperalgesiaofratmodelswithneuropathicpainviaupregulationofpparg |