Estimation Methods of the Point Spread Function Axial Position: A Comparative Computational Study

Estimation Methods of the Point Spread Function Axial Position: A Comparative Computational Study

The precise knowledge of the point spread function is central for any imaging system characterization. In fluorescence microscopy, point spread function (PSF) determination has become a common and obligatory task for each new experimental device, mainly due to its strong dependence on acquisition co...

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Bibliographic Details
Main Authors: Javier Eduardo Diaz Zamboni, Víctor Hugo Casco
Format: Article
Language:English
Published: MDPI AG 2017-01-01
Series:Journal of Imaging
Subjects:
fluorescence wide-field microscopy
optical sectioning
three-dimensional point spread function
inverse problems
parameter estimation
maximum likelihood
non-linear least square
Csiszár I-divergence
accuracy
precision
Cramer–Rao lower bound
Online Access:http://www.mdpi.com/2313-433X/3/1/7

Internet

http://www.mdpi.com/2313-433X/3/1/7

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