A Phosphite Dehydrogenase Variant with Promiscuous Access to Nicotinamide Cofactor Pools Sustains Fast Phosphite-Dependent Growth of Transplastomic <i>Chlamydomonas reinhardtii</i>

A Phosphite Dehydrogenase Variant with Promiscuous Access to Nicotinamide Cofactor Pools Sustains Fast Phosphite-Dependent Growth of Transplastomic <i>Chlamydomonas reinhardtii</i>

Heterologous expression of the NAD<sup>+</sup>-dependent phosphite dehydrogenase (PTXD) bacterial enzyme from <i>Pseudomonas stutzerii</i> enables selective growth of transgenic organisms by using phosphite as sole phosphorous source. Combining phosphite fertilization with nu...

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Bibliographic Details
Main Authors: Edoardo Cutolo, Matteo Tosoni, Simone Barera, Luis Herrera-Estrella, Luca Dall’Osto, Roberto Bassi
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Plants
Subjects:
phosphite dehydrogenase (PTXD)
<i>Chlamydomonas reinhardtii</i>
chloroplast genome engineering
algal cultivation
culture contamination prevention
nicotinamide adenine dinucleotide
Online Access:https://www.mdpi.com/2223-7747/9/4/473

Internet

https://www.mdpi.com/2223-7747/9/4/473

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