A cost-effective method for Illumina small RNA-Seq library preparation using T4 RNA ligase 1 adenylated adapters

A cost-effective method for Illumina small RNA-Seq library preparation using T4 RNA ligase 1 adenylated adapters

<p>Abstract</p> <p>Background</p> <p>Deep sequencing is a powerful tool for novel small RNA discovery. Illumina small RNA sequencing library preparation requires a pre-adenylated 3’ end adapter containing a 5’,5’-adenyl pyrophosphoryl moiety. In the absence of ATP, this...

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Bibliographic Details
Main Authors: Chen Yun-Ru, Zheng Yi, Liu Bao, Zhong Silin, Giovannoni Jim, Fei Zhangjun
Format: Article
Language:English
Published: BMC 2012-09-01
Series:Plant Methods
Subjects:
Small RNA sequencing
Directional mRNA sequencing
3’ RNA adapter
Adenylation
T4 RNA ligase 1
Online Access:http://www.plantmethods.com/content/8/1/41

Internet

http://www.plantmethods.com/content/8/1/41

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