Study of hesperetin effect on modulating transcription levels of MLH1 and MSH2 genes in SKBR3 breast cancer cell line
Hesperetin (HSP), a flavonoid, has been validated to modify gene expression and function as an epigenetic agent to stop the development of breast carcinoma cells. HSP was investigated in this research to evaluate the expression of the MLH1 and MSH2 genes in cancerous breast cell lines (SKBR3) and he...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
| Subjects: | |
| Online Access: | http://www.japtr.org/article.asp?issn=2231-4040;year=2023;volume=14;issue=4;spage=338;epage=344;aulast=Saleh |
| _version_ | 1797222493223649280 |
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| author | Naser Hameed Saleh Ahmed Salim Kadhim Al-Khafaji Esmaeil Babaei |
| author_facet | Naser Hameed Saleh Ahmed Salim Kadhim Al-Khafaji Esmaeil Babaei |
| author_sort | Naser Hameed Saleh |
| collection | DOAJ |
| description | Hesperetin (HSP), a flavonoid, has been validated to modify gene expression and function as an epigenetic agent to stop the development of breast carcinoma cells. HSP was investigated in this research to evaluate the expression of the MLH1 and MSH2 genes in cancerous breast cell lines (SKBR3) and healthy cell lines (MCF-11A) after exposure to different dosages (200, 400, and 600 μM/mL) of HSP. After 48 h of exposure, SKBR3's half-maximal inhibitory concentration was 289.6 μM/mL and MCF-10A's was 855.4 μM/mL. The research found that increasing HSP concentrations were closely correlated with an increase in MLH1 gene levels in the SKBR3 cell line, as shown by median and percentile values. HSP therapy caused the MLH1 gene expression to substantially vary in different groups, and in the SKBR3 cell line, MSH2 gene expressions were elevated in a dose-escalating manner. Moreover, HSP also raised the number of apoptotic cells, with the fraction of apoptotic cells escalating substantially at doses of 400 and 600 μM/mL. The outcomes suggested that HSP has the potential to be utilized as a therapeutic intervention for breast cancer, as it can induce apoptosis and reduce cell viability. |
| first_indexed | 2024-04-24T13:22:12Z |
| format | Article |
| id | doaj.art-0f24c29ed2584f35a87665ace5b86c1b |
| institution | Directory Open Access Journal |
| issn | 2231-4040 0976-2094 |
| language | English |
| last_indexed | 2024-04-24T13:22:12Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj.art-0f24c29ed2584f35a87665ace5b86c1b2024-04-04T11:28:38ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942023-01-0114433834410.4103/JAPTR.JAPTR_278_23Study of hesperetin effect on modulating transcription levels of MLH1 and MSH2 genes in SKBR3 breast cancer cell lineNaser Hameed SalehAhmed Salim Kadhim Al-KhafajiEsmaeil BabaeiHesperetin (HSP), a flavonoid, has been validated to modify gene expression and function as an epigenetic agent to stop the development of breast carcinoma cells. HSP was investigated in this research to evaluate the expression of the MLH1 and MSH2 genes in cancerous breast cell lines (SKBR3) and healthy cell lines (MCF-11A) after exposure to different dosages (200, 400, and 600 μM/mL) of HSP. After 48 h of exposure, SKBR3's half-maximal inhibitory concentration was 289.6 μM/mL and MCF-10A's was 855.4 μM/mL. The research found that increasing HSP concentrations were closely correlated with an increase in MLH1 gene levels in the SKBR3 cell line, as shown by median and percentile values. HSP therapy caused the MLH1 gene expression to substantially vary in different groups, and in the SKBR3 cell line, MSH2 gene expressions were elevated in a dose-escalating manner. Moreover, HSP also raised the number of apoptotic cells, with the fraction of apoptotic cells escalating substantially at doses of 400 and 600 μM/mL. The outcomes suggested that HSP has the potential to be utilized as a therapeutic intervention for breast cancer, as it can induce apoptosis and reduce cell viability.http://www.japtr.org/article.asp?issn=2231-4040;year=2023;volume=14;issue=4;spage=338;epage=344;aulast=Saleh3-(45-dimethylthiazol-2-yl)-25-diphenyltetrazolium bromide testapoptosiscytotoxicityflow cytometryhalf-maximal inhibitory concentration 50hesperetinmalignancymlh1 gene expressionmsh2 gene expression |
| spellingShingle | Naser Hameed Saleh Ahmed Salim Kadhim Al-Khafaji Esmaeil Babaei Study of hesperetin effect on modulating transcription levels of MLH1 and MSH2 genes in SKBR3 breast cancer cell line Journal of Advanced Pharmaceutical Technology & Research 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide test apoptosis cytotoxicity flow cytometry half-maximal inhibitory concentration 50 hesperetin malignancy mlh1 gene expression msh2 gene expression |
| title | Study of hesperetin effect on modulating transcription levels of MLH1 and MSH2 genes in SKBR3 breast cancer cell line |
| title_full | Study of hesperetin effect on modulating transcription levels of MLH1 and MSH2 genes in SKBR3 breast cancer cell line |
| title_fullStr | Study of hesperetin effect on modulating transcription levels of MLH1 and MSH2 genes in SKBR3 breast cancer cell line |
| title_full_unstemmed | Study of hesperetin effect on modulating transcription levels of MLH1 and MSH2 genes in SKBR3 breast cancer cell line |
| title_short | Study of hesperetin effect on modulating transcription levels of MLH1 and MSH2 genes in SKBR3 breast cancer cell line |
| title_sort | study of hesperetin effect on modulating transcription levels of mlh1 and msh2 genes in skbr3 breast cancer cell line |
| topic | 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide test apoptosis cytotoxicity flow cytometry half-maximal inhibitory concentration 50 hesperetin malignancy mlh1 gene expression msh2 gene expression |
| url | http://www.japtr.org/article.asp?issn=2231-4040;year=2023;volume=14;issue=4;spage=338;epage=344;aulast=Saleh |
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