Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells
To investigate whether complexes of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) can exert protective effects on lipopolysaccharide (LPS)-induced Caco-2 inflammatory cells, as well as the influence of high hydrostatic pressure (HHP) processing on the activities of the complexes, for determin...
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Format: | Article |
Language: | English |
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Elsevier
2021-11-01
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Series: | Journal of Functional Foods |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621003583 |
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author | Chang Tan Yanwen Kong Yuqi Tong Haotian Deng Mingyue Wang Yang Zhao Meizhi Wan Sixu Lin Xiaoxu Liu Xianjun Meng Yan Ma |
author_facet | Chang Tan Yanwen Kong Yuqi Tong Haotian Deng Mingyue Wang Yang Zhao Meizhi Wan Sixu Lin Xiaoxu Liu Xianjun Meng Yan Ma |
author_sort | Chang Tan |
collection | DOAJ |
description | To investigate whether complexes of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) can exert protective effects on lipopolysaccharide (LPS)-induced Caco-2 inflammatory cells, as well as the influence of high hydrostatic pressure (HHP) processing on the activities of the complexes, for determining their anti-apoptosis in vitro. LPS-induced Caco-2 cells were treated with C3G-BP complexes with or without HHP treatment. Results showed that C3G-BP complexes (100–100 µg/mL) inhibited the depolarization of mitochondria and reduced the production of ROS, as demonstrated by the reduced mRNA expression of IL-1β, TNF-α, and IL-8; increased expression of IL-10; and reduced iNOS, COX-2, Bcl-2, and cleaved caspase-3 levels. These findings reveal that C3G-BP exerts anti-apoptosis effects by inhibiting several mediators and cytokines involved in the inflammatory process. Furthermore, HHP-treated C3G-BP complexes better reduced cell apoptosis than untreated complexes, owing to the high pressure-mediated alterations in the monosaccharide composition of the complexes. This study provides insights for the functional changes of anthocynin-pectin complexes during food processing applications. |
first_indexed | 2024-12-13T22:51:54Z |
format | Article |
id | doaj.art-0f3d001b7f0a483582d6f02244b8d2d0 |
institution | Directory Open Access Journal |
issn | 1756-4646 |
language | English |
last_indexed | 2024-12-13T22:51:54Z |
publishDate | 2021-11-01 |
publisher | Elsevier |
record_format | Article |
series | Journal of Functional Foods |
spelling | doaj.art-0f3d001b7f0a483582d6f02244b8d2d02022-12-21T23:28:36ZengElsevierJournal of Functional Foods1756-46462021-11-0186104709Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cellsChang Tan0Yanwen Kong1Yuqi Tong2Haotian Deng3Mingyue Wang4Yang Zhao5Meizhi Wan6Sixu Lin7Xiaoxu Liu8Xianjun Meng9Yan Ma10College of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, China; Corresponding authors.Center of Experiment Teaching, Shenyang Normal University, Shenyang, Liaoning 110034, China; Corresponding authors.To investigate whether complexes of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) can exert protective effects on lipopolysaccharide (LPS)-induced Caco-2 inflammatory cells, as well as the influence of high hydrostatic pressure (HHP) processing on the activities of the complexes, for determining their anti-apoptosis in vitro. LPS-induced Caco-2 cells were treated with C3G-BP complexes with or without HHP treatment. Results showed that C3G-BP complexes (100–100 µg/mL) inhibited the depolarization of mitochondria and reduced the production of ROS, as demonstrated by the reduced mRNA expression of IL-1β, TNF-α, and IL-8; increased expression of IL-10; and reduced iNOS, COX-2, Bcl-2, and cleaved caspase-3 levels. These findings reveal that C3G-BP exerts anti-apoptosis effects by inhibiting several mediators and cytokines involved in the inflammatory process. Furthermore, HHP-treated C3G-BP complexes better reduced cell apoptosis than untreated complexes, owing to the high pressure-mediated alterations in the monosaccharide composition of the complexes. This study provides insights for the functional changes of anthocynin-pectin complexes during food processing applications.http://www.sciencedirect.com/science/article/pii/S1756464621003583High hydrostatic pressureCyanidin-3-glucosideBlueberry pectinComplexesLipopolysaccharideAnti-apoptosis |
spellingShingle | Chang Tan Yanwen Kong Yuqi Tong Haotian Deng Mingyue Wang Yang Zhao Meizhi Wan Sixu Lin Xiaoxu Liu Xianjun Meng Yan Ma Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells Journal of Functional Foods High hydrostatic pressure Cyanidin-3-glucoside Blueberry pectin Complexes Lipopolysaccharide Anti-apoptosis |
title | Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells |
title_full | Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells |
title_fullStr | Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells |
title_full_unstemmed | Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells |
title_short | Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells |
title_sort | anti apoptotic effects of high hydrostatic pressure treated cyanidin 3 glucoside and blueberry pectin complexes on lipopolysaccharide induced inflammation in caco 2 cells |
topic | High hydrostatic pressure Cyanidin-3-glucoside Blueberry pectin Complexes Lipopolysaccharide Anti-apoptosis |
url | http://www.sciencedirect.com/science/article/pii/S1756464621003583 |
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