Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells

To investigate whether complexes of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) can exert protective effects on lipopolysaccharide (LPS)-induced Caco-2 inflammatory cells, as well as the influence of high hydrostatic pressure (HHP) processing on the activities of the complexes, for determin...

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Main Authors: Chang Tan, Yanwen Kong, Yuqi Tong, Haotian Deng, Mingyue Wang, Yang Zhao, Meizhi Wan, Sixu Lin, Xiaoxu Liu, Xianjun Meng, Yan Ma
Format: Article
Language:English
Published: Elsevier 2021-11-01
Series:Journal of Functional Foods
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464621003583
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author Chang Tan
Yanwen Kong
Yuqi Tong
Haotian Deng
Mingyue Wang
Yang Zhao
Meizhi Wan
Sixu Lin
Xiaoxu Liu
Xianjun Meng
Yan Ma
author_facet Chang Tan
Yanwen Kong
Yuqi Tong
Haotian Deng
Mingyue Wang
Yang Zhao
Meizhi Wan
Sixu Lin
Xiaoxu Liu
Xianjun Meng
Yan Ma
author_sort Chang Tan
collection DOAJ
description To investigate whether complexes of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) can exert protective effects on lipopolysaccharide (LPS)-induced Caco-2 inflammatory cells, as well as the influence of high hydrostatic pressure (HHP) processing on the activities of the complexes, for determining their anti-apoptosis in vitro. LPS-induced Caco-2 cells were treated with C3G-BP complexes with or without HHP treatment. Results showed that C3G-BP complexes (100–100 µg/mL) inhibited the depolarization of mitochondria and reduced the production of ROS, as demonstrated by the reduced mRNA expression of IL-1β, TNF-α, and IL-8; increased expression of IL-10; and reduced iNOS, COX-2, Bcl-2, and cleaved caspase-3 levels. These findings reveal that C3G-BP exerts anti-apoptosis effects by inhibiting several mediators and cytokines involved in the inflammatory process. Furthermore, HHP-treated C3G-BP complexes better reduced cell apoptosis than untreated complexes, owing to the high pressure-mediated alterations in the monosaccharide composition of the complexes. This study provides insights for the functional changes of anthocynin-pectin complexes during food processing applications.
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spelling doaj.art-0f3d001b7f0a483582d6f02244b8d2d02022-12-21T23:28:36ZengElsevierJournal of Functional Foods1756-46462021-11-0186104709Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cellsChang Tan0Yanwen Kong1Yuqi Tong2Haotian Deng3Mingyue Wang4Yang Zhao5Meizhi Wan6Sixu Lin7Xiaoxu Liu8Xianjun Meng9Yan Ma10College of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, China; Corresponding authors.Center of Experiment Teaching, Shenyang Normal University, Shenyang, Liaoning 110034, China; Corresponding authors.To investigate whether complexes of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) can exert protective effects on lipopolysaccharide (LPS)-induced Caco-2 inflammatory cells, as well as the influence of high hydrostatic pressure (HHP) processing on the activities of the complexes, for determining their anti-apoptosis in vitro. LPS-induced Caco-2 cells were treated with C3G-BP complexes with or without HHP treatment. Results showed that C3G-BP complexes (100–100 µg/mL) inhibited the depolarization of mitochondria and reduced the production of ROS, as demonstrated by the reduced mRNA expression of IL-1β, TNF-α, and IL-8; increased expression of IL-10; and reduced iNOS, COX-2, Bcl-2, and cleaved caspase-3 levels. These findings reveal that C3G-BP exerts anti-apoptosis effects by inhibiting several mediators and cytokines involved in the inflammatory process. Furthermore, HHP-treated C3G-BP complexes better reduced cell apoptosis than untreated complexes, owing to the high pressure-mediated alterations in the monosaccharide composition of the complexes. This study provides insights for the functional changes of anthocynin-pectin complexes during food processing applications.http://www.sciencedirect.com/science/article/pii/S1756464621003583High hydrostatic pressureCyanidin-3-glucosideBlueberry pectinComplexesLipopolysaccharideAnti-apoptosis
spellingShingle Chang Tan
Yanwen Kong
Yuqi Tong
Haotian Deng
Mingyue Wang
Yang Zhao
Meizhi Wan
Sixu Lin
Xiaoxu Liu
Xianjun Meng
Yan Ma
Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells
Journal of Functional Foods
High hydrostatic pressure
Cyanidin-3-glucoside
Blueberry pectin
Complexes
Lipopolysaccharide
Anti-apoptosis
title Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells
title_full Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells
title_fullStr Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells
title_full_unstemmed Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells
title_short Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells
title_sort anti apoptotic effects of high hydrostatic pressure treated cyanidin 3 glucoside and blueberry pectin complexes on lipopolysaccharide induced inflammation in caco 2 cells
topic High hydrostatic pressure
Cyanidin-3-glucoside
Blueberry pectin
Complexes
Lipopolysaccharide
Anti-apoptosis
url http://www.sciencedirect.com/science/article/pii/S1756464621003583
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