Expression and Purification of the Arabidopsis E4 SUMO Ligases PIAL1 and PIAL2
The proteins PIAL1 (At1g08910) and PIAL2 (At5g41580) are members of the recently discovered group of plant E4 SUMO ligases. This protocol allows quick and simple expression of the recombinant proteins in Escherichia coli (E. coli) and subsequent affinity purification using a maltose binding protein...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2015-08-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=1544&type=0 |