Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach

Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach

Summary: These protocols investigate the interaction of cytonemes with localized Wnt. Cell-niche signaling between naive or primed mouse embryonic stem cells (ESCs) and either Wnt-secreting trophoblast stem cells (TSCs) or Wnt signals tethered to microbeads can be scrutinized in vitro. This approach...

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Bibliographic Details
Main Authors: Sergi Junyent, Joshua Reeves, Shukry J. Habib
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Developmental biology
Microscopy
Signal Transduction
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005190

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http://www.sciencedirect.com/science/article/pii/S2666166721005190

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