Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals
Advances in spectral deconvolution technologies are rapidly enabling researchers to replace or enhance traditional epifluorescence microscopes with instruments capable of detecting numerous markers simultaneously in a multiplexed fashion. While significantly expediting sample throughput and elucidat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2011-11-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/11/11/10557/ |
| _version_ | 1798005739205165056 |
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| author | Sara G. Becker-Catania Zachary E. St. Louis Guoliang Tao Robert C. Triulzi Travis L. Jennings |
| author_facet | Sara G. Becker-Catania Zachary E. St. Louis Guoliang Tao Robert C. Triulzi Travis L. Jennings |
| author_sort | Sara G. Becker-Catania |
| collection | DOAJ |
| description | Advances in spectral deconvolution technologies are rapidly enabling researchers to replace or enhance traditional epifluorescence microscopes with instruments capable of detecting numerous markers simultaneously in a multiplexed fashion. While significantly expediting sample throughput and elucidating sample information, this technology is limited by the spectral width of common fluorescence reporters. Semiconductor nanocrystals (NC’s) are very bright, narrow band fluorescence emitters with great potential for multiplexed fluorescence detection, however the availability of NC’s with facile attachment chemistries to targeting molecules has been a severe limitation to the advancement of NC technology in applications such as immunocytochemistry and immunohistochemistry. Here we report the development of simple, yet novel attachment chemistries for antibodies onto NC’s and demonstrate how spectral deconvolution technology enables the multiplexed detection of 5 distinct NC-antibody conjugates with fluorescence emission wavelengths separated by as little as 20 nm. |
| first_indexed | 2024-04-11T12:43:52Z |
| format | Article |
| id | doaj.art-0f9e781d08a14105a2651ae2f975d88b |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T12:43:52Z |
| publishDate | 2011-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-0f9e781d08a14105a2651ae2f975d88b2022-12-22T04:23:25ZengMDPI AGSensors1424-82202011-11-011111105571057010.3390/s111110557Simplistic Attachment and Multispectral Imaging with Semiconductor NanocrystalsSara G. Becker-CataniaZachary E. St. LouisGuoliang TaoRobert C. TriulziTravis L. JenningsAdvances in spectral deconvolution technologies are rapidly enabling researchers to replace or enhance traditional epifluorescence microscopes with instruments capable of detecting numerous markers simultaneously in a multiplexed fashion. While significantly expediting sample throughput and elucidating sample information, this technology is limited by the spectral width of common fluorescence reporters. Semiconductor nanocrystals (NC’s) are very bright, narrow band fluorescence emitters with great potential for multiplexed fluorescence detection, however the availability of NC’s with facile attachment chemistries to targeting molecules has been a severe limitation to the advancement of NC technology in applications such as immunocytochemistry and immunohistochemistry. Here we report the development of simple, yet novel attachment chemistries for antibodies onto NC’s and demonstrate how spectral deconvolution technology enables the multiplexed detection of 5 distinct NC-antibody conjugates with fluorescence emission wavelengths separated by as little as 20 nm.http://www.mdpi.com/1424-8220/11/11/10557/quantum dotnanocrystalIHCimmunohistochemistryspectral imagingmultiplexingconjugationantibody |
| spellingShingle | Sara G. Becker-Catania Zachary E. St. Louis Guoliang Tao Robert C. Triulzi Travis L. Jennings Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals Sensors quantum dot nanocrystal IHC immunohistochemistry spectral imaging multiplexing conjugation antibody |
| title | Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals |
| title_full | Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals |
| title_fullStr | Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals |
| title_full_unstemmed | Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals |
| title_short | Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals |
| title_sort | simplistic attachment and multispectral imaging with semiconductor nanocrystals |
| topic | quantum dot nanocrystal IHC immunohistochemistry spectral imaging multiplexing conjugation antibody |
| url | http://www.mdpi.com/1424-8220/11/11/10557/ |
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