Controlled-rate freezing of human ES cells
A significant obstacle to using human embryonic stem cells (hESCs) arises from extremely poor survival associated with freezing, typically in the range of 1%. This report describes a slow controlled-rate freezing technique commonly used for mammalian embryo cryopreservation. Using a combination of s...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2005-06-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/05386ST01 |