Combining phage display with SMRTbell next-generation sequencing for the rapid discovery of functional scFv fragments

Combining phage display with SMRTbell next-generation sequencing for the rapid discovery of functional scFv fragments

Phage display technology in combination with next-generation sequencing (NGS) currently is a state-of-the-art method for the enrichment and isolation of monoclonal antibodies from diverse libraries. However, the current NGS methods employed for sequencing phage display libraries are limited by the s...

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Bibliographic Details
Main Authors: Francesco Nannini, Lenart Senicar, Farhaan Parekh, Khai J. Kong, Alexander Kinna, Reyisa Bughda, James Sillibourne, Xihao Hu, Biao Ma, Yuchen Bai, Mathieu Ferrari, Martin A. Pule, Shimobi C. Onuoha
Format: Article
Language:English
Published: Taylor & Francis Group 2021-01-01
Series:mAbs
Subjects:
Antibody discovery
rat immune libraries
phage display
long-read sequencing
scFv antibody
affinity modulation
Online Access:https://www.tandfonline.com/doi/10.1080/19420862.2020.1864084

Internet

https://www.tandfonline.com/doi/10.1080/19420862.2020.1864084

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