Optimized Protocol for Protein Extraction from the Breast Tissue that is Compatible with Two-Dimensional Gel Electrophoresis

Proteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge to use a tissue for proteomics studies is the small sample size and difficulties to extract and preserve proteins. The choice of a buffer compatible with proteomics applications is...

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Bibliographic Details
Main Authors: Olena Zakharchenko, Christina Greenwood, Louise Alldridge, Serhiy Souchelnytskyi
Format: Article
Language:English
Published: SAGE Publishing 2011-01-01
Series:Breast Cancer: Basic and Clinical Research
Online Access:https://doi.org/10.4137/BCBCR.S6263