3D Printed Monolithic Microreactors for Real-Time Detection of <em>Klebsiella pneumoniae</em> and the Resistance Gene <em>bla</em><sub>NDM-1</sub> by Recombinase Polymerase Amplification

3D Printed Monolithic Microreactors for Real-Time Detection of <em>Klebsiella pneumoniae</em> and the Resistance Gene <em>bla</em><sub>NDM-1</sub> by Recombinase Polymerase Amplification

We investigate the compatibility of three 3D printing materials towards real-time recombinase polymerase amplification (rtRPA). Both the general ability of the rtRPA reaction to occur while in contact with the cured 3D printing materials as well as the residual autofluorescence and fluorescence drif...

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Bibliographic Details
Main Authors: Ole Behrmann, Matthias Hügle, Franz Eckardt, Iris Bachmann, Cecilia Heller, Marina Schramm, Carrie Turner, Frank T. Hufert, Gregory Dame
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:Micromachines
Subjects:
recombinase polymerase amplification (RPA)
antibiotic resistance
3D printing
Online Access:https://www.mdpi.com/2072-666X/11/6/595

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https://www.mdpi.com/2072-666X/11/6/595

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