Targeting DNA repair pathways with B02 and Nocodazole small molecules to improve CRIS-PITCh mediated cassette integration in CHO-K1 cells

Targeting DNA repair pathways with B02 and Nocodazole small molecules to improve CRIS-PITCh mediated cassette integration in CHO-K1 cells

Abstract CRISPR-mediated integration could be used to develop the recombinant CHO (rCHO) cells by knock-in into the hotspot loci. However, low HDR efficiency besides the complex donor design is the main barrier for achieving so. The recently introduced MMEJ-mediated CRISPR system (CRIS-PITCh) uses a...

Full description

Bibliographic Details
Main Authors: Behnaz Rahmani, Mohammad Hassan Kheirandish, Samaneh Ghanbari, Abbasali Raz, Fahimeh Shamsi, Fatemeh Davami
Format: Article
Language:English
Published: Nature Portfolio 2023-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-29863-8

Internet

https://doi.org/10.1038/s41598-023-29863-8

Similar Items