Molecular Diagnosis of Leishmaniasis in Spain: Development and Validation of Ready-To-Use Gel-Form Nested and Real-Time PCRs To Detect Leishmania spp.

Molecular Diagnosis of Leishmaniasis in Spain: Development and Validation of Ready-To-Use Gel-Form Nested and Real-Time PCRs To Detect Leishmania spp.

ABSTRACT Leishmaniasis is an endemic parasitic disease in at least 98 countries. In Spain, it is considered a zoonosis caused by Leishmania infantum, with an annual incidence of 0.62 cases/100,000 inhabitants. The predominant clinical manifestations are the cutaneous (CL) and visceral forms (VL), an...

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Bibliographic Details
Main Authors: Carmen Chicharro, Javier Nieto, Silvia Miguelañez, Emilia Garcia, Sheila Ortega, Ana Peña, Jose Miguel Rubio, Maria Flores-Chavez
Format: Article
Language:English
Published: American Society for Microbiology 2023-06-01
Series:Microbiology Spectrum
Subjects:
leishmaniasis
molecular diagnosis
sensitivity
specificity
nested PCR
qPCR
Online Access:https://journals.asm.org/doi/10.1128/spectrum.03354-22

Internet

https://journals.asm.org/doi/10.1128/spectrum.03354-22

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