Two-Step Purification of L-Asparaginase from Acrylaway® L
Abstract L-Asparaginase (L-ASNase) is a biopharmaceutical used for acute lymphoblastic leukaemia (ALL) treatment, dramatically increasing the patients’ chance of cure. However, its production and distribution in developing countries were disrupted because of its low profitability, which caused great...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2022-08-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100666&tlng=en |
| _version_ | 1828324165901352960 |
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| author | Louise Lacalendola Tundisi Diego de Freitas Coêlho Alessandra Valéria de Sousa Faria Adalberto Pessoa Junior Elias Basile Tambourgi Laura de Oliveira Nascimento Edgar Silveira Priscila Gava Mazzola |
| author_facet | Louise Lacalendola Tundisi Diego de Freitas Coêlho Alessandra Valéria de Sousa Faria Adalberto Pessoa Junior Elias Basile Tambourgi Laura de Oliveira Nascimento Edgar Silveira Priscila Gava Mazzola |
| author_sort | Louise Lacalendola Tundisi |
| collection | DOAJ |
| description | Abstract L-Asparaginase (L-ASNase) is a biopharmaceutical used for acute lymphoblastic leukaemia (ALL) treatment, dramatically increasing the patients’ chance of cure. However, its production and distribution in developing countries were disrupted because of its low profitability, which caused great concern among patients. This study evaluates the feasibility of combining fractional precipitation and aqueous two-phase systems (ATPS) to purify L-ASNase from a low-grade product, commercially known as Acrylaway® L. The ATPS purification results were not particularly expressive compared to the two-step purification process composed of ethanol precipitation and gel filtration, which was able to recover the target molecule with a purification factor over 5 fold. Thus, we studied a purification process capable of manufacturing pharmaceutical grade L-ASNase from a commercially available low-grade raw material; however, improvements regarding its throughput must be achieved, and high purity is the first step to apply it as a new biopharmaceutical product. The proposed process could pose as a short-time solution to mitigate its shortage while a cost-effective production plant is being developed. |
| first_indexed | 2024-04-13T19:02:04Z |
| format | Article |
| id | doaj.art-102ccfa4a5024dcd80c6e5ae31d7466a |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-13T19:02:04Z |
| publishDate | 2022-08-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-102ccfa4a5024dcd80c6e5ae31d7466a2022-12-22T02:34:03ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902022-08-015810.1590/s2175-97902022e191042Two-Step Purification of L-Asparaginase from Acrylaway® LLouise Lacalendola Tundisihttps://orcid.org/0000-0002-3374-6103Diego de Freitas Coêlhohttps://orcid.org/0000-0002-2123-5891Alessandra Valéria de Sousa Fariahttps://orcid.org/0000-0003-1161-5724Adalberto Pessoa Juniorhttps://orcid.org/0000-0002-5268-8690Elias Basile Tambourgihttps://orcid.org/0000-0003-3275-0810Laura de Oliveira Nascimentohttps://orcid.org/0000-0002-4741-8493Edgar Silveirahttps://orcid.org/0000-0001-7249-639XPriscila Gava Mazzolahttps://orcid.org/0000-0002-3795-8189Abstract L-Asparaginase (L-ASNase) is a biopharmaceutical used for acute lymphoblastic leukaemia (ALL) treatment, dramatically increasing the patients’ chance of cure. However, its production and distribution in developing countries were disrupted because of its low profitability, which caused great concern among patients. This study evaluates the feasibility of combining fractional precipitation and aqueous two-phase systems (ATPS) to purify L-ASNase from a low-grade product, commercially known as Acrylaway® L. The ATPS purification results were not particularly expressive compared to the two-step purification process composed of ethanol precipitation and gel filtration, which was able to recover the target molecule with a purification factor over 5 fold. Thus, we studied a purification process capable of manufacturing pharmaceutical grade L-ASNase from a commercially available low-grade raw material; however, improvements regarding its throughput must be achieved, and high purity is the first step to apply it as a new biopharmaceutical product. The proposed process could pose as a short-time solution to mitigate its shortage while a cost-effective production plant is being developed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100666&tlng=enAqueous two-phases systemL-AsparaginaseDownstream processesBioprocessingFractional precipitation |
| spellingShingle | Louise Lacalendola Tundisi Diego de Freitas Coêlho Alessandra Valéria de Sousa Faria Adalberto Pessoa Junior Elias Basile Tambourgi Laura de Oliveira Nascimento Edgar Silveira Priscila Gava Mazzola Two-Step Purification of L-Asparaginase from Acrylaway® L Brazilian Journal of Pharmaceutical Sciences Aqueous two-phases system L-Asparaginase Downstream processes Bioprocessing Fractional precipitation |
| title | Two-Step Purification of L-Asparaginase from Acrylaway® L |
| title_full | Two-Step Purification of L-Asparaginase from Acrylaway® L |
| title_fullStr | Two-Step Purification of L-Asparaginase from Acrylaway® L |
| title_full_unstemmed | Two-Step Purification of L-Asparaginase from Acrylaway® L |
| title_short | Two-Step Purification of L-Asparaginase from Acrylaway® L |
| title_sort | two step purification of l asparaginase from acrylaway r l |
| topic | Aqueous two-phases system L-Asparaginase Downstream processes Bioprocessing Fractional precipitation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100666&tlng=en |
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