CCL3 Enhances Antitumor Immune Priming in the Lymph Node via IFNγ with Dependency on Natural Killer Cells
Lymph node (LN) plays a critical role in tumor cell survival outside of the primary tumor sites and dictates overall clinical response in many tumor types (1, 2). Previously, we and others have demonstrated that CCL3 plays an essential role in orchestrating T cell—antigen-presenting cell (APC) encou...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01390/full |
| _version_ | 1818874805644951552 |
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| author | Frederick Allen Peter Rauhe David Askew Alexander A. Tong Joseph Nthale Saada Eid Jay T. Myers Caryn Tong Alex Y. Huang Alex Y. Huang Alex Y. Huang Alex Y. Huang |
| author_facet | Frederick Allen Peter Rauhe David Askew Alexander A. Tong Joseph Nthale Saada Eid Jay T. Myers Caryn Tong Alex Y. Huang Alex Y. Huang Alex Y. Huang Alex Y. Huang |
| author_sort | Frederick Allen |
| collection | DOAJ |
| description | Lymph node (LN) plays a critical role in tumor cell survival outside of the primary tumor sites and dictates overall clinical response in many tumor types (1, 2). Previously, we and others have demonstrated that CCL3 plays an essential role in orchestrating T cell—antigen-presenting cell (APC) encounters in the draining LN following vaccination, and such interactions enhance the magnitude of the memory T cell pool (3–5). In the current study, we investigate the cellular responses in the tumor-draining lymph nodes (TDLNs) of a CCL3-secreting CT26 colon tumor (L3TU) as compared to wild-type tumor (WTTU) during the priming phase of an antitumor response (≤10 days). In comparison to WTTU, inoculation of L3TU resulted in suppressed tumor growth, a phenomenon that is accompanied by altered in vivo inflammatory responses on several fronts. Autologous tumor-derived CCL3 (aCCL3) secretion by L3TU bolstered the recruitment of T- and B-lymphocytes, tissue-migratory CD103+ dendritic cells (DCs), and CD49b+ natural killer (NK) cells, resulting in significant increases in the differentiation and activation of multiple Interferon-gamma (IFNγ)-producing leukocytes in the TDLN. During this early phase of immune priming, NK cells constitute the major producers of IFNγ in the TDLN. CCL3 also enhances CD8+ T cell proliferation and differentiation by augmenting DC capacity to drive T cell activation in the TDLN. Our results revealed that CCL3-dependent IFNγ production and CCL3-induced DC maturation drive the priming of effective antitumor immunity in the TDLN. |
| first_indexed | 2024-12-19T13:16:26Z |
| format | Article |
| id | doaj.art-1038f084ce7d45e09a05e4d5d2e89fce |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-19T13:16:26Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-1038f084ce7d45e09a05e4d5d2e89fce2022-12-21T20:19:49ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-10-01810.3389/fimmu.2017.01390308390CCL3 Enhances Antitumor Immune Priming in the Lymph Node via IFNγ with Dependency on Natural Killer CellsFrederick Allen0Peter Rauhe1David Askew2Alexander A. Tong3Joseph Nthale4Saada Eid5Jay T. Myers6Caryn Tong7Alex Y. Huang8Alex Y. Huang9Alex Y. Huang10Alex Y. Huang11Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH, United StatesDepartment of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, United StatesDepartment of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, United StatesDepartment of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH, United StatesDepartment of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, United StatesDepartment of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, United StatesDepartment of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, United StatesDepartment of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, United StatesDepartment of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH, United StatesDepartment of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, United StatesAngie Fowler AYA Cancer Institute, UH Rainbow Babies & Children’s Hospital, Cleveland, OH, United StatesCase Comprehensive Cancer Center, Cleveland, OH, United StatesLymph node (LN) plays a critical role in tumor cell survival outside of the primary tumor sites and dictates overall clinical response in many tumor types (1, 2). Previously, we and others have demonstrated that CCL3 plays an essential role in orchestrating T cell—antigen-presenting cell (APC) encounters in the draining LN following vaccination, and such interactions enhance the magnitude of the memory T cell pool (3–5). In the current study, we investigate the cellular responses in the tumor-draining lymph nodes (TDLNs) of a CCL3-secreting CT26 colon tumor (L3TU) as compared to wild-type tumor (WTTU) during the priming phase of an antitumor response (≤10 days). In comparison to WTTU, inoculation of L3TU resulted in suppressed tumor growth, a phenomenon that is accompanied by altered in vivo inflammatory responses on several fronts. Autologous tumor-derived CCL3 (aCCL3) secretion by L3TU bolstered the recruitment of T- and B-lymphocytes, tissue-migratory CD103+ dendritic cells (DCs), and CD49b+ natural killer (NK) cells, resulting in significant increases in the differentiation and activation of multiple Interferon-gamma (IFNγ)-producing leukocytes in the TDLN. During this early phase of immune priming, NK cells constitute the major producers of IFNγ in the TDLN. CCL3 also enhances CD8+ T cell proliferation and differentiation by augmenting DC capacity to drive T cell activation in the TDLN. Our results revealed that CCL3-dependent IFNγ production and CCL3-induced DC maturation drive the priming of effective antitumor immunity in the TDLN.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01390/fullCCL3natural killer cellsCD103+ dendritic cellslymphocytesinterferon-gammalymph node |
| spellingShingle | Frederick Allen Peter Rauhe David Askew Alexander A. Tong Joseph Nthale Saada Eid Jay T. Myers Caryn Tong Alex Y. Huang Alex Y. Huang Alex Y. Huang Alex Y. Huang CCL3 Enhances Antitumor Immune Priming in the Lymph Node via IFNγ with Dependency on Natural Killer Cells Frontiers in Immunology CCL3 natural killer cells CD103+ dendritic cells lymphocytes interferon-gamma lymph node |
| title | CCL3 Enhances Antitumor Immune Priming in the Lymph Node via IFNγ with Dependency on Natural Killer Cells |
| title_full | CCL3 Enhances Antitumor Immune Priming in the Lymph Node via IFNγ with Dependency on Natural Killer Cells |
| title_fullStr | CCL3 Enhances Antitumor Immune Priming in the Lymph Node via IFNγ with Dependency on Natural Killer Cells |
| title_full_unstemmed | CCL3 Enhances Antitumor Immune Priming in the Lymph Node via IFNγ with Dependency on Natural Killer Cells |
| title_short | CCL3 Enhances Antitumor Immune Priming in the Lymph Node via IFNγ with Dependency on Natural Killer Cells |
| title_sort | ccl3 enhances antitumor immune priming in the lymph node via ifnγ with dependency on natural killer cells |
| topic | CCL3 natural killer cells CD103+ dendritic cells lymphocytes interferon-gamma lymph node |
| url | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01390/full |
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