Real-time monitoring of rhamnose induction effect on the expression of mpt64 gene fused with pelB signal peptide in Escherichia coli BL21 (DE3)

Real-time monitoring of rhamnose induction effect on the expression of mpt64 gene fused with pelB signal peptide in Escherichia coli BL21 (DE3)

In this research, Escherichia coli BL21 (DE3) harboring an expression vector constructed with a rhamnose-inducible promoter and a pelB signal peptide was used as a host cell to produce MPT64 protein. The objective of this research was to figure out the optimum time of mpt64 gene expression through r...

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Bibliographic Details
Main Authors: Sri Agung Fitri Kusuma, Ida Parwati, Toto Subroto, Yaya Rukayadi, Tina Rostinawati, Muhammad Yusuf, Muhammad Fadhlillah, Laily D Tanti, Risa R Ahyudanari
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2020-01-01
Series:Journal of Advanced Pharmaceutical Technology & Research
Subjects:
escherichia coli bl21 (de3)
mpt64
pelb
real time
rhamnose
Online Access:http://www.japtr.org/article.asp?issn=2231-4040;year=2020;volume=11;issue=2;spage=69;epage=73;aulast=Kusuma

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http://www.japtr.org/article.asp?issn=2231-4040;year=2020;volume=11;issue=2;spage=69;epage=73;aulast=Kusuma

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