Versatile protein tagging in cells with split fluorescent protein

Versatile protein tagging in cells with split fluorescent protein

Tagging proteins with fluorescent proteins is a powerful method for both imaging and non-imaging applications. Here the authors use the eleventh β-strand of sfGFP and sfCherry as epitope tags for multicolour imaging and amplified signals by tandem arrangement; shortness of the tag enabled introducti...

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Bibliographic Details
Main Authors: Daichi Kamiyama, Sayaka Sekine, Benjamin Barsi-Rhyne, Jeffrey Hu, Baohui Chen, Luke A. Gilbert, Hiroaki Ishikawa, Manuel D. Leonetti, Wallace F. Marshall, Jonathan S. Weissman, Bo Huang
Format: Article
Language:English
Published: Nature Portfolio 2016-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms11046

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https://doi.org/10.1038/ncomms11046

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